Detection of anticentromere antibodies using recombinant human CENP-A protein.
Detection of anticentromere antibodies using recombinant human CENP-A protein.
复制标题
使用重组人 CENP-A 蛋白检测抗着丝粒抗体。
DOI：
10.1002/art.1780390520
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发表时间：
1996
影响因子：
--
通讯作者：
Hoch,SO
中科院分区：
文献类型：
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作者：
Sun,D;Martinez,A;Sullivan,KF;Sharp,GC;Hoch,SO
目的：使用重组蛋白（rCENP‐A）评估CENP‐A与抗着丝点抗体（ACA）的反应性。方法：使用杆状病毒系统在昆虫细胞中过表达人CENP‐A抗原。我们通过免疫印迹和酶联免疫吸附试验（ELISA）检测ACA对全长重组多肽的活性。结果：在研究的ACA+血清（n = 38）中，在ELISA系统中检测rCENP‐A时，95%为阳性。在ACA血清（n = 100）中，仅2%的检测试剂盒产生假阳性结果。重组抗原与真实抗原在ACA反应性测定中具有良好的相关性。结论：CENP‐A是一个重要的ACA靶点。rCENP‐A检测试剂盒的可用性是先前描述的rCENP‐B检测试剂盒在ACA临床意义分析中的有价值辅助。
Objective.To evaluate CENP‐A reactivity with anticentromere antibodies (ACA) using recombinant protein (rCENP‐A).Methods.Human CENP‐A antigen was overexpressed in insect cells using the baculovirus system. We tested for ACA activity against the full‐length recombinant polypeptide by immunoblot and by enzyme‐linked immunosorbent assay (ELISA).Results.Of the ACA+ sera studied (n = 38), 95% were positive when tested against the rCENP‐A in the ELISA system. Of the ACA‐ sera (n = 100), only 2% gave false‐positive results in the assay. There was good correlation between the recombinant and bona fide antigens in assaying for ACA reactivity.Conclusion.CENP‐A is a significant ACA target. The availability of the rCENP‐A assay is a valuable adjunct to the previously described rCENP‐B assay in analyses of the clinical significance of ACA.