Lens epithelial apoptosis and cell proliferation in human age-related cortical cataract
Lens epithelial apoptosis and cell proliferation in human age-related cortical cataract
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DOI：
10.1177/112067210501500206
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发表时间：
2005-03-01
影响因子：
1.7
通讯作者：
Delides, G
中科院分区：
文献类型：
--
作者：
Charakidas, A;Kalogeraki, A;Delides, G
目的。探讨年龄相关性皮质性白内障人晶状体上皮细胞凋亡的存在，并评估细胞增殖，这是凋亡细胞死亡的预测结果，在这一特定细胞群中。采用末端地高高素标记的dUTP缺口末端标记（TUNEL），对接受成人皮质性白内障囊外摘除术（n=27）或接受透明晶状体摘除术以矫正高度近视（n=25）的患者的囊切标本进行DNA片段化评估。在23个白内障晶状体上皮细胞和20个非白内障晶状体上皮细胞中检测细胞增殖标记物MIB1，这是一种针对核抗原Ki-67的单克隆抗体，在整个细胞周期中检测到，但在静息（GO）细胞中不存在。在25例（92.6%）白内障晶状体中观察到TUNEL染色，而在2例（8%）非白内障晶状体上皮中发现细胞凋亡。仅检测到2例mib1阳性标本，其中1例为白内障囊内摘出术中获得的一颗囊。人皮质性白内障晶状体上皮细胞凋亡率低。这种有限的上皮细胞凋亡不太可能导致任何显著的细胞密度下降，因为上皮间隙很可能被前晶状体囊萌发区的细胞增殖所取代。然而，在一生中，小范围上皮细胞损失的积累可能导致晶状体纤维形成和体内平衡的改变，并导致晶状体透明度的丧失。
PURPOSE. To probe the presence of apoptosis in the epithelium of human lenses with age-related cortical cataract as well as to assess cell proliferation, a predicted consequence of apoptotic cell death, in this specific cell population.METHODS. DNA fragmentation was assessed using terminal digoxigenin-labeled dUTP nick end labeling (TUNEL) in capsulotomy specimens obtained from patients who underwent either extracapsular cataract extraction for the removal of adult-onset cortical cataract (n=27) or clear lens extraction for the correction of high myopia (n=25). Cell proliferation was assayed in 23 epithelia of cataractous lenses, and 20 epithelia of non-cataractous lenses with the proliferation marker MIB1, a monoclonal antibody against the nuclear antigen Ki-67 that is detected throughout the cell cycle but is absent in the resting (GO) cell.RESULTS. TUNEL staining was observed in 25 (92.6%) specimens of cataractous lenses, whereas cells undergoing apoptosis were identified in 2 (8%) of the epithelia from non-cataractous lenses. Only two MIB1-positive samples were detected, one of which was a capsule obtained during intracapsular cataract extraction.CONCLUSIONS. The epithelium of human lenses with cortical cataract undergoes low rate apoptotic death. This limited epithelial apoptosis is unlikely to result in any significant cell density decrease since epithelial gaps are likely to be replaced by cell proliferation at the germinative zone of the anterior lens capsule. Nevertheless, the accumulation of small-scale epithelial losses during lifetime may induce alterations in lens fiber formation and homeostasis and result in loss of lens transparency.