Chloroquine inhibited Ebola virus replication in vitro but failed to protect against infection and disease in the in vivo guinea pig model
Chloroquine inhibited Ebola virus replication in vitro but failed to protect against infection and disease in the in vivo guinea pig model
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DOI：
10.1099/jgv.0.000309
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发表时间：
2015-12-01
影响因子：
3.8
通讯作者：
Carroll, Miles W.
中科院分区：
文献类型：
--
作者：
Dowall, Stuart D.;Bosworth, Andrew;Carroll, Miles W.
埃博拉病毒（EBOV）是高致病性的，具有在人群中引起暴发并伴随显著死亡的倾向。由于缺乏经批准的治疗方法，已开展了筛选可能有效药物的方案。这些研究之一已经使用假型化测定证明了氯喹针对EBOV的可能效用。在EBOV疾病的小鼠模型中，关于氯喹的治疗效果存在相互矛盾的报道。目前还没有使用更大和更严格的豚鼠感染模型的疗效报告。在这项研究中，我们已经表明，活的EBOV的复制在体外受到氯喹的损害。然而，在体内没有观察到保护作用时，EBOV感染的豚鼠用氯喹治疗。这些结果表明，氯喹不应被视为EBOV的治疗策略。
Ebola virus (EBOV) is highly pathogenic, with a predisposition to cause outbreaks in human populations accompanied by significant mortality. Owing to the lack of approved therapies, screening programmes of potentially efficacious drugs have been undertaken. One of these studies has demonstrated the possible utility of chloroquine against EBOV using pseudotyped assays. In mouse models of EBOV disease there are conflicting reports of the therapeutic effects of chloroquine. There are currently no reports of its efficacy using the larger and more stringent guinea pig model of infection. In this study we have shown that replication of live EBOV is impaired by chloroquine in vitro. However, no protective effects were observed in vivo when EBOV-infected guinea pigs were treated with chloroquine. These results advocate that chloroquine should not be considered as a treatment strategy for EBOV.