Exogenous Cell Surface Modification with Cell Penetrating Peptide-Conjugated Lipids Causes Spontaneous Cell Adhesion
Exogenous Cell Surface Modification with Cell Penetrating Peptide-Conjugated Lipids Causes Spontaneous Cell Adhesion
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DOI：
10.1021/acsabm.1c00335
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发表时间：
2021-04-30
影响因子：
4.7
通讯作者：
Teramura, Yuji
中科院分区：
文献类型：
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作者：
Noiri, Makoto;Goto, Yuya;Teramura, Yuji
在基底上的细胞图案化技术对于基于细胞的测定中的平台非常重要。通常使用阳离子聚合物、细胞外基质和抗体对基底进行化学处理以用于细胞图案化。然而，如果有一种方法可以在不处理衬底表面的情况下对电池进行处理，则电池图案化可能会更容易。我们以前报道过，细胞粘附可以诱导细胞表面的细胞穿透肽（CPP）共轭聚（乙二醇）-磷脂（CPP-PEG-脂质）的修改。这种方法不需要对基底表面（例如聚苯乙烯或玻璃）进行化学改性，并且可以用于浮动细胞的细胞图案化。在这里，我们的目的是研究诱导细胞粘附的机制，使用代表性的CPP，达特肽（Tat-PEG-lipid）。我们发现，细胞粘附诱导通过静电之间的相互作用的达特肽和基板表面，这可以更有效地诱导通过增加分子量的PEG与CPPs，但不与阳离子肽。相邻PEG链之间的排斥体积效应可以比具有较低分子量的PEG更好地拉伸细胞形状，使细胞牢固地铺展。此外，Tat-PEG-lipid不激活肌动蛋白丝的形成，也不影响黏着斑激酶的表达。因此，CPP-PEG-脂质诱导的细胞粘附不影响内部细胞信号传导。
The technique of cell patterning on a substrate is of great importance for platforms in cell-based assays. Chemical treatment of the substrate is commonly performed for cell patterning using cationic polymers, extracellular matrices, and antibodies. However, cell patterning could be easier if there is an approach to immobilize cells without treating the substrate surface. We previously reported that cell adhesion could be induced by the modification of the cellular surface with a cell-penetrating peptide (CPP)-conjugated poly(ethylene glycol)-phospholipid (CPP-PEG-lipid). This approach does not require chemical modification of the substrate surface, such as polystyrene or glass, and can be used for the cell patterning of floating cells. Here, we aimed to study the mechanism of induced cell adhesion using a representative CPP, Tat peptide (Tat-PEG-lipid). We found that cell adhesion was induced via electrostatic interactions between the Tat peptide and the substrate surface, which could be induced more efficiently by increasing the molecular weight of PEG together with CPPs but not with cationic peptides. The excluded volume effect between neighboring PEG chains could stretch the cell shape better than PEG with lower molecular weight, allowing the cell to spread firmly. In addition, Tat-PEG-lipid did not activate actin filament formation and did not influence the expression of focal adhesion kinase. Thus, the induced cell adhesion by CPP-PEG-lipid did not affect internal cell signaling.