Potential of ancestral sylvatic dengue-2 viruses to re-emerge
Potential of ancestral sylvatic dengue-2 viruses to re-emerge
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DOI：
10.1016/j.virol.2006.08.049
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发表时间：
2007-02-20
期刊：
影响因子：
3.7
通讯作者：
Weaver, Scott C.
中科院分区：
文献类型：
--
作者：
Vasilakis, Nikos;Shell, Elisabeth J.;Weaver, Scott C.
登革病毒（DENY）是世界上热带和亚热带地区最重要的虫媒病毒病原体。DENV传播包括非人灵长类动物和伊蚊属树栖蚊子之间的森林性、地方性循环，以及埃及伊蚊（一种在生活区周围的水容器中具有幼虫发育的蚊子）和人储库宿主之间的城市性、地方性/流行性循环。地方性DENY的所有4种血清型都独立于祖先森林病毒进化而来，并且在生态和进化上都变得不同;这一过程可能涉及对（i）栖息地周围蚊子媒介和/或（ii）人类储存宿主的适应。为了检验后一种假设，我们评估了森林型和地方性DENV-2毒株（代表来自东南亚、西非和美洲的主要基因型）在两种替代人类模型宿主中复制的能力：单核细胞衍生的人树突状细胞（moDCs）和植入人肝癌细胞的小鼠。虽然各种DENV-2菌株在两种模型中的平均复制滴度显示出显著的菌株间差异，但在任一模型中均未检测到森林型和地方性菌株之间的总体差异。我们的研究结果表明，地方性登革病毒株从祖先的森林毒株中出现可能不需要适应，以更有效地在人类宿主中复制，这意味着森林登革病毒株重新出现进入地方性循环的可能性很高。共享的复制配置文件的美国地方性和森林菌株表明，美国菌株保持或恢复祖先的表型。(c)2006年爱思唯尔公司All rights reserved.
Dengue viruses (DENY) are the most important arboviral pathogens in tropical and subtropical regions throughout the world. DENV transmission includes both a sylvatic, enzootic cycle between nonhuman primates and arboreal mosquitoes of the genus Aedes, and an urban, endemic/epidemic cycle between Aedes aegypti, a mosquito with larval development in peridomestic water containers, and human reservoir hosts. All 4 serotypes of endemic DENY evolved independently from ancestral sylvatic viruses and have become both ecologically and evolutionarily distinct; this process may have involved adaptation to (i) peridomestic mosquito vectors and/or (ii) human reservoir hosts. To test the latter hypothesis, we assessed the ability of sylvatic and endemic DENV-2 strains, representing major genotypes from Southeast Asia, West Africa and the Americas, to replicate in two surrogate human model hosts: monocyte-derived, human dendritic cells (moDCs), and mice engrafted with human hepatoma cells. Although the various DENV-2 strains showed significant inter-strain variation in mean replication titers in both models, no overall difference between sylvatic and endemic strains was detected in either model. Our findings suggest that emergence of endemic DENV strains from ancestral sylvatic strains may not have required adaptation to replicate more efficiently in human reservoir hosts, implying that the potential for re-emergence of sylvatic dengue strains into the endemic cycle is high. The shared replication profiles of the American endemic and sylvatic strains suggest that American strains have maintained or regained the ancestral phenotype. (c) 2006 Elsevier Inc. All rights reserved.