3D bioprinting for engineering complex tissues.
3D bioprinting for engineering complex tissues.
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DOI：
10.1016/j.biotechadv.2015.12.011
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发表时间：
2016-07
影响因子：
16
通讯作者：
Kim DH
中科院分区：
文献类型：
--
作者：
Mandrycky C;Wang Z;Kim K;Kim DH
生物打印是一种3D制造技术，用于精确分配载有细胞的生物材料，以构建复杂的3D功能活组织或人工器官。尽管仍处于早期阶段，但生物打印策略已经证明了其在再生医学中的潜在用途，以生成各种可移植组织，包括皮肤、软骨和骨。然而，目前的生物打印方法在复杂3D组织内的高分辨率细胞沉积、受控细胞分布、血管化和神经支配方面仍存在技术挑战。虽然没有一种通用的生物打印方法出现，但它仍然是一种按需的多功能制造技术，可以解决日益严重的器官短缺问题，并为广泛的生物医学工程应用提供微米级细胞图案化的高通量方法。本文介绍了生物打印的基本原理、材料、集成策略和应用。我们还讨论了用于工程化复杂组织的3D生物打印的最新发展，当前挑战和未来前景。结合人类多能干细胞技术的最新进展，3D生物打印组织模型可以作为高通量预测药物筛选和更有效再生疗法的支持平台。
Bioprinting is a 3D fabrication technology used to precisely dispense cell-laden biomaterials for the construction of complex 3D functional living tissues or artificial organs. While still in its early stages, bioprinting strategies have demonstrated their potential use in regenerative medicine to generate a variety of transplantable tissues, including skin, cartilage, and bone. However, current bioprinting approaches still have technical challenges in terms of high-resolution cell deposition, controlled cell distributions, vascularization, and innervation within complex 3D tissues. While no one-size-fits-all approach to bioprinting has emerged, it remains an on-demand, versatile fabrication technique that may address the growing organ shortage as well as provide a high-throughput method for cell patterning at the micrometer scale for broad biomedical engineering applications. In this review, we introduce the basic principles, materials, integration strategies and applications of bioprinting. We also discuss the recent developments, current challenges and future prospects of 3D bioprinting for engineering complex tissues. Combined with recent advances in human pluripotent stem cell technologies, 3D-bioprinted tissue models could serve as an enabling platform for high-throughput predictive drug screening and more effective regenerative therapies.