Effects of different dietary levels of fish protein hydrolysates on growth, digestive enzymes, gut microbiota, and resistance to Vibrio anguillarum in European sea bass (Dicentrarchus labrax) larvae
Effects of different dietary levels of fish protein hydrolysates on growth, digestive enzymes, gut microbiota, and resistance to Vibrio anguillarum in European sea bass (Dicentrarchus labrax) larvae
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DOI：
10.1016/j.cbpa.2006.12.037
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发表时间：
2007-05-01
影响因子：
2.3
通讯作者：
Cahu, C.
中科院分区：
文献类型：
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作者：
Kotzamanis, Y. P.;Gisbert, E.;Cahu, C.
将两种鱼蛋白水解物（FPH）添加到四种为开始饲养的黑鲈幼鱼准备的饲料中，分别添加两种不同水平（占总成分的10%和19%）：一种是商业FPH CPSP，其中可溶性肽的主要部分分子质量（51%）在500-2500 Da之间，另一种是沙丁鱼内脏酸性青贮得到的实验FPH SH，可溶性肽的主要部分（54%）在200 - 500 Da之间。通过观察毛囊边缘膜上消化酶（碱性磷酸酶和氨基肽酶N）的早期活性，得出饲粮中添加10%商品FPH在生长、存活和肠道发育方面的最佳效果。这与C10组幼虫中弧菌含量较低有关。高剂量的FPH，特别是在富含短肽的实验制剂中，似乎有利于Vibrio sp. TYH3的优势，其表现为机会性的。实验FPH的效果尚不明确，因为早期幼虫对鳗弧菌的抗性更强，特别是在高FPH剂量（S19组）下。这可能是由于鳗弧菌与TYH3弧菌之间的直接拮抗作用，或者是刺激了幼虫的免疫反应。综上所述，不同分子量组分和浓度的饲料可溶性肽可能影响黑鲈幼鱼的生长性能和免疫状态。因此，低剂量的商业FPH似乎是可取的，无论是对幼虫的发育还是对细菌环境，尽管需要进一步的研究来确定和表征可能对油脂生长和免疫反应有有益影响的肽部分，并确定它们在海鲈鱼幼虫的饲料中的最佳添加水平。(c) 2007爱思唯尔公司版权所有。
Two fish protein hydrolysates (FPH) were incorporated into four diets prepared for start-feeding sea bass larvae, at two different levels (10% and 19% of total ingredients): a commercial FPH, CPSP, in which the molecular mass of the main fraction of soluble peptides (51%) was between 500-2500 Da, and an experimental FPH obtained by acidic silage of sardine offal, SH, with a main portion of soluble peptides (54%) ranging from 200 to 500 Da. The diet with 10% of the commercial FPH gave the best results in terms of growth, survival and intestinal development, as evaluated by the early activity of digestive enzymes in the brush border membrane (alkaline phosphatase and aminopeptidase N). This was related to the low level of Vibrio spp. counted in the larvae of group C10. The high dose of FPH, especially in the experimental preparation rich in short peptides, seemed to favour the dominance of Vibrio sp. TYH3, which behaved opportunistically. The effect of the experimental FPH was ambiguous, since early larvae challenged with Vibrio anguillarum were more resistant to the pathogen, especially at high FPH dose (group S19). This might be due either to direct antagonism between V anguillarum and Vibrio sp. TYH3, or to the stimulation of the immune response in the larvae. These results indicate that different molecular weight fractions and concentrations of feed-soluble peptides may affect the growth performance and immunological status of sea bass larvae. Consequently, a low dose of commercial FPH seems advisable, both for larval development and for the bacterial environment, although further research is required to determine and characterize peptide fractions that may have a beneficial effect oil growth and immune response, and to determine their optimal inclusion levels in diets for sea bass larvae. (c) 2007 Elsevier Inc. All rights reserved.