Kinetic effects of inorganic pyrophosphate analogs on several inorganic pyrophosphate hydrolyzing enzymes.
Kinetic effects of inorganic pyrophosphate analogs on several inorganic pyrophosphate hydrolyzing enzymes.
复制标题
无机焦磷酸类似物对几种无机焦磷酸水解酶的动力学影响。
DOI：
10.1021/bi00741a029
复制
发表时间：
1973
期刊：
影响因子：
2.9
通讯作者：
L. Butler
中科院分区：
文献类型：
--
作者：
S. Kelly;F. Feldman;J. W. Sperow;L. Butler
Susan J. Kelly, Fred Feldman, James W. Sperow和Larry G. Butler*摘要：在无机焦磷酸盐类似物中，桥氧被氮（亚胺二磷酸）或碳（亚甲基二膦酸盐）取代，引起动力学反应，从微粒体无机焦磷酸酶的完全互换性到玉米叶片中有机焦磷酸酶完全缺乏对类似物的识别。来自大肠杆菌和小牛肠道的非特异性碱性磷酸酶都能水解亚胺二磷酸，但水解PPj的速率要低得多。这两种酶对4-硝基苯基磷酸的水解均有较高的竞争性抑制
Susan J. Kelly, Fred Feldman, James W. Sperow, and Larry G. Butler* abstract: Inorganic pyrophosphate analogs in which the bridge oxygen is replaced by nitrogen (imidodiphosphate) or carbon (methylene diphosphonate) elicit kinetic responses which vary from complete interchangeability of imidodiphos-phate and PPi by microsomal inorganic pyrophosphatase to total lack of recognition of the analogs by a maize leaf in-organic pyrophosphatase. Nonspecific alkaline phosphatases from Escherichia coli and from calf intestine both hydrolyze imidodiphosphate, but at much lower rates than they hy-drolyze PPj. Hydrolysis of 4-nitrophenyl phosphate by both these enzymes was competitively inhibited at relatively high