Evidence that catalysis by yeast inorganic pyrophosphatase proceeds by direct phosphoryl transfer to water and not via a phosphoryl enzyme intermediate.
Evidence that catalysis by yeast inorganic pyrophosphatase proceeds by direct phosphoryl transfer to water and not via a phosphoryl enzyme intermediate.
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有证据表明，酵母无机焦磷酸酶的催化作用是通过直接磷酰基转移到水中而不是通过磷酰基酶中间体进行的。
DOI：
10.1021/bi00300a002
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Cooperman,BS
中科院分区：
文献类型：
--
作者：
Gonzalez,MA;Webb,MR;Welsh,KM;Cooperman,BS
方法ATPyS和SPX水解。用高效液相色谱法测定atp水解率，然后测定atp的消失率（Eccleston & Webb, 1982）。注射前用0.1 mL HPLC洗脱缓冲液（0.7 M磷酸铵，pH 4.0）淬灭含有1 nmol核苷酸的等分液。采用改良的Fiske-Subbarow法测定P的形成，以测定PPase催化SP水解的速率（Josse, 1966）。
Methods ATPyS and SPX Hydrolysis. Rates of ATPyS hydrolysis were followed by measuring ATPyS disappearance by HPLC (Eccleston & Webb, 1982). Aliquots containing 1 nmol of nucleotide were quenched prior to injection with 0.1 mL of HPLC eluting buffer (0.7 M ammonium phosphate, pH 4.0). Rates of PPase catalysis of SP¡ hydrolysis were followed by measuring P¡ formation using a modified Fiske-Subbarow method (Josse, 1966).