Effect of non-surgical periodontal treatment on clinical parameters and the numbers of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans at adult periodontitis sites
Effect of non-surgical periodontal treatment on clinical parameters and the numbers of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans at adult periodontitis sites
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DOI：
10.1034/j.1600-051x.2001.028005437.x
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发表时间：
2001-05-01
影响因子：
6.7
通讯作者：
Douglas, CWI
中科院分区：
文献类型：
--
作者：
Doungudomdacha, S;Rawlinson, A;Douglas, CWI
背景、目标：本研究的目的是相关的牙龈卟啉单胞菌，中间普氏菌和放线菌伴放线杆菌细胞的数量与临床参数在牙周病和健康的网站之前和之后的非手术牙周治疗使用敏感的定量PCR方法（Q-PCR）。从50名成人牙周炎患者的541个部位收集治疗前、治疗后和随访时（治疗后3-6个月）的龈下菌斑样本。结果：定量分析显示，牙龈卟啉单胞菌计数与牙周袋深度（p=0.006）和附着丧失（p=0.010）相关，而中间卟啉单胞菌和A.伴随放线菌与临床症状相关。治疗后，患病部位和健康部位的所有三种菌的数量均显著减少（86-99%），但无一被根除。治疗前和治疗后研究的三个物种中的任何两个之间都发现了正相关性。通过随访，深部部位的探测深度有明显改善（p=0.001），但没有其他临床参数。这项研究证明了Q-PCR用于计数临床牙周标本中的假定病原体，并且所有部位的三种微生物的数量随着非手术牙周治疗而减少。
Background, aims: The purpose of this study was to relate the numbers of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans cells to clinical parameters at diseased and healthy periodontal sites before and after non-surgical periodontal therapy using a sensitive quantitative PCR method (Q-PCR).Method: The sensitivity of the Q-PCR was less than 10 cells for all three species. Subgingival plaque samples R-ere collected from 541 sites in 50 adult periodontitis subjects pre-treatment, post-treatment and at a follow-up visit (3-6 months posttreatment). Pocket probing depth, attachment loss and bleeding on probing were recorded at each visit and both healthy and diseased sites in each subject were sampled.Results: Quantification revealed that P, gingivalis counts were associated with pocket depth (p=0.006) and attachment loss (p=0.010); however, neither P. intermedia nor A. actinomycetemcomitans was associated with the clinical signs examined, Post-treatment, there was a significant decrease in the numbers of all three species in both the diseased and healthy sites (86-99%) but none were eradicated. Positive associations were found between any two of the three species studied both pre- and post-therapy By the follow-up visit, there was a significant improvement in the probing depth of deep sites (p=0.001) but in no other clinical parameters.Conclusion: This study demonstrates the usefulness of Q-PCR for enumerating putative pathogens in clinical periodontal specimens and that the numbers of the three organisms in all sites decrease with non-surgical periodontal therapy.