Cellulase Activity in Blood Cockle ( Anadara granosa ) in the Matang Mangrove Forest Reserve, Malaysia
Cellulase Activity in Blood Cockle ( Anadara granosa ) in the Matang Mangrove Forest Reserve, Malaysia
复制标题
马来西亚马当红树林保护区血蚶(Anadara granosa)的纤维素酶活性
DOI:
10.6090/jarq.46.355
复制
发表时间:
2012
影响因子:
0.4
通讯作者:
Katsuhisa Tanaka
中科院分区:
文献类型:
--
作者:
Takatoshi Niiyama;H. Toyohara;Katsuhisa Tanaka
To validate the utilization of organic matter, mainly composed of cellulose derived from mangrove trees by bivalves, we analyzed the cellulase activity in blood cockles (Anadara granosa) in the Mantang Mangrove Forest Reserve, Malaysia. In an agar plate assay, unstained circles due to cellulase activity were clearly detected in the extract of blood cockle digestive organs. Additionally, SDS-PAGE zymographic analysis revealed intensive active bands at 9.5 kDa and weak smear bands above 24.5 kDa. These results clearly showed that the blood cockle, the dominant mangrove bivalve species, possesses cellulase activity used to break down cellulose derived from mangrove trees. The present paper first reports the occurrence of the cellulose breakdown function in a mangrove bivalve, blood cockle, via biochemical analyses and suggests the possible ecological function of this species in the mangrove food chain. Discipline: Fisheries Additional key words: agar plate assay, cellulose, digestive organs, zymography
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
K. Sakamoto;K. Touhata;A. Kasai;H. Toyohara
通讯作者:
H. Toyohara
DOI:
--
发表时间:
2007
期刊:
Biochimie 89
影响因子:
--
作者:
Y. Nishida;K. Suzuki;Y. kumagai;H. Tanaka;A. Inoue;T. Ojima
通讯作者:
T. Ojima