Cellulase Activity in Blood Cockle ( Anadara granosa ) in the Matang Mangrove Forest Reserve, Malaysia

Cellulase Activity in Blood Cockle ( Anadara granosa ) in the Matang Mangrove Forest Reserve, Malaysia
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马来西亚马当红树林保护区血蚶(Anadara granosa)的纤维素酶活性

DOI:
10.6090/jarq.46.355
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发表时间:
2012
影响因子:
0.4
通讯作者:
Katsuhisa Tanaka
Katsuhisa Tanaka
中科院分区:
农林科学4区
文献类型:
--
作者:
Takatoshi Niiyama;H. Toyohara;Katsuhisa Tanaka

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为了验证双壳类对红树林中主要由纤维素组成的有机质的利用,我们分析了马来西亚曼塘红树林保护区的血螺(Anadara Ranosa)的纤维素酶活性。在琼脂平板试验中,在血吸虫消化器官提取液中可清楚地检测到纤维素酶活性所致的未染色圆圈。此外,SDS-PAGE酶谱分析显示,在9.5 kDa处有较强的活性条带,而在24.5 kDa以上的涂片条带较弱。这些结果清楚地表明,红树林双壳类中的优势种血贝具有分解红树纤维素的纤维素酶活性。本文通过生化分析首次报道了红树林双壳类血贝的纤维素分解功能,并提出了该物种在红树林食物链中可能的生态功能。学科:渔业新增关键词:琼脂平板分析、纤维素、消化器官、酶谱
To validate the utilization of organic matter, mainly composed of cellulose derived from mangrove trees by bivalves, we analyzed the cellulase activity in blood cockles (Anadara granosa) in the Mantang Mangrove Forest Reserve, Malaysia. In an agar plate assay, unstained circles due to cellulase activity were clearly detected in the extract of blood cockle digestive organs. Additionally, SDS-PAGE zymographic analysis revealed intensive active bands at 9.5 kDa and weak smear bands above 24.5 kDa. These results clearly showed that the blood cockle, the dominant mangrove bivalve species, possesses cellulase activity used to break down cellulose derived from mangrove trees. The present paper first reports the occurrence of the cellulose breakdown function in a mangrove bivalve, blood cockle, via biochemical analyses and suggests the possible ecological function of this species in the mangrove food chain. Discipline: Fisheries Additional key words: agar plate assay, cellulose, digestive organs, zymography
DOI: --
发表时间: 2008
期刊:
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发表时间: 2007
期刊: Biochimie 89
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