Reactive oxygen species are not involved in the onset of age-related memory impairment in Drosophila
Reactive oxygen species are not involved in the onset of age-related memory impairment in Drosophila
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DOI：
10.1111/j.1601-183x.2011.00748.x
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发表时间：
2012-02-01
影响因子：
2.5
通讯作者：
Saitoe, M.
中科院分区：
文献类型：
--
作者：
Hirano, Y.;Kuriyama, Y.;Saitoe, M.
活性氧（ROS）的损伤被认为是机体衰老的原因之一。活性氧也被认为与几种年龄相关的表型有关，包括年龄相关的记忆障碍（AMI）。然而，ROS的增加是否会影响AMI的发生，此前还没有研究。在这里，我们研究了喂食过氧化氢和ros生成剂百草枯对幼年和急性心肌梗死发病期间果蝇嗅觉厌恶记忆的影响。活性氧喂养大大降低了苍蝇的存活率，增加了苍蝇头部氧化蛋白和抗氧化酶过氧化氢酶（Cat）和应激反应伴侣热休克蛋白22 （Hsp22）的转录本。然而，喂养并没有损害幼年野生型果蝇的记忆，也没有加剧AMI发病时果蝇的记忆缺陷。引人注目的是，ROS喂养确实会破坏幼年记忆，当蘑菇体（嗅觉记忆所需的神经中枢）中涉及ROS防御系统的基因（包括抗氧化酶和Hsp22）表达减少时，观察到AMI发作加速。这些结果表明，尽管ROS的产生随着年龄的增长而增加，但记忆过程所需的神经元功能即使在AMI发病时也受到防御系统对ROS的充分保护。因此，我们认为ROS的产生并不影响果蝇AMI的发病。
Damage from reactive oxygen species (ROS) is thought to be a cause of organismal aging. Reactive oxygen species have also been proposed to be responsible for several age-associated phenotypes, including age-related memory impairment (AMI). However, it has not previously been tested whether increasing ROS affects AMI onset. Here we examined the effects of feeding hydrogen peroxide, and the ROS-generating agent, paraquat, on olfactory aversive memory in Drosophila at young ages and during AMI onset. Reactive oxygen species feeding greatly reduced fly survival, and increased oxidized proteins and transcripts of an antioxidant enzyme, catalase (Cat) and a stress-responsive chaperone, heat-shock protein 22 (Hsp22) in fly heads. However, feeding did not impair memory in young wild-type flies, nor did it exacerbate the memory deficits in flies at the onset of AMI. Strikingly ROS feeding did disrupt memory at young ages and accelerated AMI onset was observed when expression of genes involved in the defense system to ROS, including antioxidant enzymes and Hsp22, was reduced in the mushroom bodies, neural centers required for olfactory memory. These results implicate that although ROS production increases upon aging, neuronal functions required for memory processes are sufficiently protected by the defense system to ROS even at the age of AMI onset. Thus we propose that ROS production does not affect AMI onset in Drosophila.