Anti-oxidation activity of the crude polysaccharides isolated from Polygonum Cillinerve (Nakai) Ohwi in immunosuppressed mice
Anti-oxidation activity of the crude polysaccharides isolated from Polygonum Cillinerve (Nakai) Ohwi in immunosuppressed mice
复制标题
从Polygonum Cillinerve (Nakai) Ohwi 中分离的粗多糖对免疫抑制小鼠的抗氧化活性
DOI：
10.1016/j.jep.2010.08.052
复制
发表时间：
2010-11-11
影响因子：
5.4
通讯作者：
Zhang, Zhi-Qi
中科院分区：
文献类型：
--
作者：
Cui, Jun-Jian;Yuan, Jiang-Feng;Zhang, Zhi-Qi
研究目的：Polygonum Cillinerve (Nakai) Ohwi 在中国已被广泛使用 2000 多年。前期研究表明，从Polygonum Cillinerve (Nakai) Ohwi (PCCP)中提取的粗多糖具有清除自由基和体外抗肿瘤活性。本研究进一步探讨了PCCP在免疫抑制小鼠中的抗氧化作用。方法和材料：ICR小鼠首先给予环磷酰胺(CY，150mg/kg)，1天后，用不同剂量的PCCP或盐水溶液治疗，每天1次，共21天。最后一次给药二十四小时后，对动物进行称重，然后斩首处死。检测肝、脾、胸腺指数，并评价各组织（肝、心、肾）的生化参数。结果：灌胃PCCP能够克服环磷酰胺引起的免疫抑制，并显着提高TOC、CAT、SOD和GSH-Px水平。它还可以提高小鼠的肝脏、脾脏和胸腺指数，降低MDA水平。结论：PCCP具有显着的自由基清除和抗氧化活性，在预防免疫系统氧化损伤中发挥重要作用。 (C) 2010 Elsevier Ireland Ltd. 保留所有权利。
Aim of the study: Polygonum Cillinerve (Nakai) Ohwi is commonly used in China for over 2000 years. Previous research has shown that the crude polysaccharides extracted from Polygonum Cillinerve (Nakai) Ohwi (PCCP) have the scavenging free radicals and anti-tumor activities in vitro. In present study, PCCP were further approached the perspective of their anti-oxidation in immunosuppressed mice.Methods and materials: ICR mice were treated firstly with cyclophosphamide (CY, 150 mg/kg), 1 day later, treated with different dosages of PCCP or saline solution once daily for 21 days. Twenty-four hours later for the last drug administration, the animals were weighed, and then killed by decapitation. The liver, spleen, and thymus indices were investigated, and the biochemical parameters were evaluated for various tissues (liver, heart, and kidney).Results: The administration of PCCP with gavage was able to overcome the cyclophosphamide-induced immunosuppression, and significantly to raise the TOC, CAT, SOD, and GSH-Px level. It also raised the liver, spleen, and thymus indices, and decreased the MDA level in mice.Conclusions: PCCP possess the pronounced free radical-scavenging and antioxidant activities, and could play an important role in the prevention of oxidative damage in immunological system. (C) 2010 Elsevier Ireland Ltd. All rights reserved.