Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase
Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase
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DOI：
10.1006/bbrc.1996.0477
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发表时间：
1996-03-27
影响因子：
3.1
通讯作者：
Sandberg, M
中科院分区：
文献类型：
--
作者：
Orstavik, S;Solberg, R;Sandberg, M
II型cGMP依赖性蛋白激酶是最初从小肠分离的酶，并且被认为参与肠离子转运和液体分泌的调节。从小脑文库中分离出编码人II型cGMP依赖性蛋白激酶的一部分的互补DNA克隆。基于来自该互补DNA的序列信息，使用聚合酶链反应从人脑信使RNA扩增II型cGMP依赖性蛋白激酶的5 '末端。复合互补DNA编码762个氨基酸的蛋白质，计算分子量为87.4 kDa。在小肠、结肠和前列腺中检测到编码II型cGMP依赖性蛋白激酶的信使RNA。通过使用聚合酶链反应和Southern杂交体细胞杂交，编码II型cGMP依赖性蛋白激酶的基因定位于人类染色体4 q13.1-q21.1。(C)出版社：Academic Press，Inc.
The type II cGMP-dependent protein kinase is an enzyme originally isolated from the small intestine, and is thought to be involved in the regulation of intestinal ion transport and fluid secretion. A complementary DNA clone encoding a part of the human type II cGMP-dependent protein kinase was isolated from a cerebellum library. Based on sequence information from this complementary DNA, the 5'-end of the type II cGMP-dependent protein kinase was amplified from human brain messenger RNA using polymerase chain reaction. The composite complementary DNA encoded a 762 amino acid protein with a calculated molecular mass of 87.4 kDa. Messenger RNAs encoding the type II cGMP-dependent protein kinase were detected in small intestine, colon and prostate. By using polymerase chain reaction and Southern blotting on somatic cell hybrids, the gene encoding the type II cGMP-dependent protein kinase was mapped to human chromosome 4 q13.1-q21.1. (C) 1996 Academic Press, Inc.