Distribution of phosphatidylethanolamine arachidonic acid in platelet membranes.
Distribution of phosphatidylethanolamine arachidonic acid in platelet membranes.
复制标题
磷脂酰乙醇胺花生四烯酸在血小板膜中的分布。
DOI：
10.1016/0005-2736(81)90362-x
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Mills,DC
中科院分区：
文献类型：
--
作者：
Schick,PK;Schick,BP;Brandeis,G;Mills,DC
测定了血小板表面磷脂酰乙醇胺（PE）中的花生四烯酸（20：4）和其他脂肪酸和醛类。通过使用2，4，6-三硝基苯磺酸盐，一种非穿透性探针（Schick，P.K.，K.B.库里卡和Chacko，G. K.（1976）J. Clin. Invest. 57，1221-1226）。PE含有50%的总血小板花生四烯酸。约16%的血小板PE存在于血小板表面。研究表明，血小板表面PE的脂肪酸和醛组成与血小板内PE的脂肪酸和醛组成几乎相同。因此，8 nmol花生四烯酸存在于109个血小板的质膜外层的PE中。
Arachidonic acid (20:4) and other fatty acids and aldehydes in phosphatidylethanolamine (PE) present on the platelet surface was determined. Surface-exposed PE was isolated by using 2,4,6-trinitrobenzenesulfonate, a nonpenetrating probe (Schick, P.K., Kurica, K.B. and Chacko, G.K. (1976) J. Clin. Invest. 57, 1221–1226). PE contains 50% total platelet arachidonic acid. Approx. 16% platelet PE is present on the platelet surface. The study showed that the fatty acid and aldehyde composition of PE on the platelet surface is virtually identical to that in PE present inside the platelet. Therefore, 8 nmol arachidonic acid are present in PE in the outer layer of the plasma membrane in 109platelets.