Single-Cell Single-Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryonic Lysates.
Single-Cell Single-Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryonic Lysates.
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DOI：
10.1007/978-1-0716-2035-9_4
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Dickinson DJ
中科院分区：
文献类型：
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作者：
Stolpner N;Dickinson DJ
Mapping how proteins form complexes and change binding partners is a central to part of understanding cell signaling. Bulk biochemistry can provide a summary of what complexes are present in a cell, but information about the diversity of individual protein complexes is lost. Here, we describe single-cell, single molecule pull down (sc-SiMPull) a TIRF microscopy-based coimmunoprecipitation method to visualize thousands of individual proteins, their binding partners, and protein complex stoichiometry directly from single cell lysate. By iterating sc-SiMPull over time, temporal dynamics of protein complexes in response to signaling can be constructed.
