The zinc finger transcription factors Bbctf1⊍ and Bbctf1⊎ regulate the expression of genes involved in lipid degradation and contribute to stress tolerance and virulence in a fungal insect pathogen
The zinc finger transcription factors Bbctf1⊍ and Bbctf1⊎ regulate the expression of genes involved in lipid degradation and contribute to stress tolerance and virulence in a fungal insect pathogen
复制标题
锌指转录因子 Bbctf1– 和 Bbctf1– 调节参与脂质降解的基因表达，并有助于真菌昆虫病原体的胁迫耐受性和毒力
DOI：
10.1002/ps.5797
复制
发表时间：
--
期刊：
影响因子：
--
通讯作者：
Yu XP
中科院分区：
文献类型：
--
作者：
Wang ZL;Pan HB;Huang J;Yu XP
为了引发昆虫感染，昆虫病原真菌产生多种角质层降解酶。其中，脂解酶参与表皮脂质水解，从而促进真菌穿透昆虫宿主的最外层表皮屏障。Far/CTF 1型锌指转录因子在调节植物病原体的脂肪分解活性和真菌致病性方面发挥重要作用，但在真菌昆虫病原体中的功能尚不清楚。结果在真菌昆虫病原体球孢白僵菌中鉴定出两种Far/CTF 1型转录因子Bbctf 1 α和Bbctf 1 β，这两种因子对于真菌脂质降解相关基因的差异表达至关重要，并进行了功能表征。每个基因的破坏导致胞外脂肪分解活性在真菌底物诱导条件下的急剧损失，随后是与真菌生物控制潜力相关的显著表型缺陷。这些缺陷主要包括菌丝生长和分生孢子形成的严重障碍，以及在正常或诱导条件下菌落生长过程中对氧化和细胞壁扰动胁迫的耐受性的急剧丧失。生物测定结果表明，各突变体对大蜡螟的毒力在局部浸泡条件下均明显减弱。然而，当绕过角质层穿透过程时，血淋巴内注射没有显着差异。结论SBbctf 1 α和Bbctf 1 β是多功能转录因子，在调节真菌脂质利用方面发挥重要作用，并有助于B的营养生长、孢子形成能力、环境适应性和害虫控制潜力。巴西纳。© 2020化学工业学会
BACKGROUNDTo initiate insect infection, entomopathogenic fungi produce diverse cuticle‐degrading enzymes. Of those, lipolytic enzymes participate in epicuticular lipid hydrolysis and thus facilitate fungal penetration through the outermost cuticular barrier of the insect host. The Far/CTF1‐type zinc finger transcription factors play an important role in the regulation of lipolytic activity and fungal pathogenicity in plant pathogens but remain functionally unknown in fungal insect pathogens.RESULTSTwo Far/CTF1‐type transcription factor Bbctf1α and Bbctf1β, which are essential for differential expression of genes involved in the fungal lipid degradation, were identified and functionally characterized in a fungal entomopathogenBeauveria bassiana. Disruption of each gene led to drastic losses of extracellular lipolytic activities under lipidic substrate‐inducing conditions, followed by remarkable phenotypic defects associated with the fungal biocontrol potential. These defects mainly included severe impairments of mycelial growth and conidium formation, and drastic losses of tolerance to the stresses of oxidation and cell wall perturbation during colony growth under either normal or induction conditions. Bioassays showed that the virulence of each disruption mutant on the greater wax moth was remarkably attenuated in topical immersion. However, there was no significant difference in intrahemolymph injection when the cuticle penetration process was bypassed.CONCLUSIONSBbctf1α and Bbctf1β are multifunctional transcription factors that play vital roles in the regulation of fungal lipid utilization and contribute to the vegetative growth, sporulation capacity, environmental fitness and pest control potential inB. bassiana. © 2020 Society of Chemical Industry