Developmental Patterns of Sleep Slow Wave Activity and Synaptic Density in Adolescent Mice
Developmental Patterns of Sleep Slow Wave Activity and Synaptic Density in Adolescent Mice
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DOI：
10.5665/sleep.3570
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发表时间：
2014-04-01
期刊：
影响因子：
5.6
通讯作者：
Cirelli, Chiara
中科院分区：
文献类型：
--
作者：
de Vivo, Luisa;Faraguna, Ugo;Cirelli, Chiara
研究目的：人类睡眠中的慢波活动 (SWA) 在青春期会下降。有人认为这种下降反映了皮质突触的消除，但这一假设从未得到直接检验。 设计：我们关注小鼠额叶皮层，收集（1）SWA从青春期早期（类似于出生后第20天，P20）到成年期（P60）的数据； (2)突触前标志物突触蛋白I的表达； （3）层 I-II 中的树突棘数量。设置：基础睡眠研究实验室。患者或参与者：YFP 系 H 小鼠（n = 70；P15-87，所有雄性）和 GFP 系 S 小鼠（n = 14；P17-60，8 只雌性）用于脑电图记录。 45 只 YFP 小鼠（P19-119，12 只雌性）和 42 只 GFP-S 小鼠（P20-60，14 只雌性）分别用于体内 2 光子成像和离体共聚焦显微镜。其他 YGP 小鼠（n = 57，P10-77）用于突触蛋白 I 的蛋白质印迹分析。干预：不适用。测量和结果：与人类一样，小鼠的 SWA 从青春期早期到成年期下降。突触蛋白 I 水平从 P10 增加到 P24，之后几乎没有变化。 V 层锥体神经元 (YFP-H) 顶端树突的平均棘密度从 P20 到 P60 没有变化。属于III层和V层锥体神经元(GFP-S)的I-II层顶端树突的棘数量从P20到P30略有增加，从P30到P60减少；从 P20 到 P60，较小的棘数量减少，而较大的棘数量增加。结论：在小鼠中，SWA 发育下降不太可能是由于皮质突触的净修剪所致。
Study Objective: In humans sleep slow wave activity (SWA) declines during adolescence. It has been suggested that this decline reflects the elimination of cortical synapses, but this hypothesis has never been tested directly.Design: We focused on mouse frontal cortex and collected data from early adolescence (similar to postnatal day 20, P20) to adulthood (P60) of (1) SWA; (2) expression of synapsin I, a presynaptic marker; and (3) number of dendritic spines in layers I-II.Setting: Basic sleep research laboratory.Patients or Participants: YFP-line H mice (n = 70; P15-87, all males) and GFP-line S mice (n = 14; P17-60, 8 females) were used for EEG recording. Forty-five YFP mice (P19-119, 12 females) and 42 GFP-S mice (P20-60, 14 females) were used for in vivo 2-photon imaging and ex vivo confocal microscopy, respectively. Other YGP mice (n = 57, P10-77) were used for western blot analysis of synapsin I.Interventions: N/A.Measurements and Results: As in humans, SWA in mice declined from early adolescence to adulthood. Synapsin I levels increased from P10 to P24, with little change afterwards. Mean spine density in apical dendrites of layer V pyramidal neurons (YFP-H) showed no change from P20 to P60. Spine number in layers I-II apical dendrites, belonging to layer III and V pyramidal neurons (GFP-S), increased slightly from P20 to P30 and decreased from P30 to P60; smaller spines decreased in number from P20 to P60, while bigger spines increased.Conclusions: In mice, it is unlikely that the developmental decrease in SWA can be accounted for by a net pruning of cortical synapses.