RB and Cdc2 expression in brain: correlations with 3H-thymidine incorporation and neurogenesis
RB and Cdc2 expression in brain: correlations with 3H-thymidine incorporation and neurogenesis
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DOI：
10.1523/jneurosci.13-07-02930.1993
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发表时间：
1993-07
期刊：
影响因子：
--
通讯作者：
HJ Okano;D. Pfaff;R. Gibbs
中科院分区：
文献类型：
--
作者：
HJ Okano;D. Pfaff;R. Gibbs
细胞周期调节蛋白Rb和p34cdc2在成年大鼠脑中的表达，特别是在海马结构和嗅球中的增殖和神经元分化。Rb样免疫反应(Rb-IR)在整个脑内均可检测到，尤其是在海马锥体细胞、梨状皮质和小脑浦肯野细胞中的表达尤为强烈。在齿状回颗粒下区和从前侧脑室延伸至嗅球的室管膜下区，Rb-IR和cdc2-IR在增殖的神经元前体细胞中也有表达。根据神经元特异性烯醇化酶(NSE)的表达和在戊四氮诱导的癫痫发作后海马区Fos-IR的表达来评估这些细胞中的许多发育成神经元。Rb-IR核强阳性的增殖细胞数与分化为功能性海马神经元的胸腺嘧啶核苷标记细胞数之间存在良好的相关性。在分化过程中，Rb-IR也显著下降，并且发生在NSE表达之前。讨论了Rb的丢失可能是神经元分化所必需的可能性。
Expression of the cell cycle regulatory proteins RB and p34cdc2 was examined in the adult rat brain, with special emphasis on proliferation and neuronal differentiation in the hippocampal formation and olfactory bulb. RB-like immunoreactivity (RB-IR) was detected throughout the brain, with particularly intense staining observed in hippocampal pyramidal cells, pyriform cortex, and cerebellar Purkinje cells. Intense RB-IR and cdc2-IR were also detected in proliferating neuronal precursor cells in the subgranular region of the dentate gyrus and in the subependymal region extending from the anterior lateral ventricle into the olfactory bulb. Many of these cells developed into neurons as assessed by the expression of neuron-specific enolase (NSE) and, in the hippocampal formation, the expression of Fos-IR following pentylenetetrazol-induced seizure activity. A good correlation was observed between the number of proliferating cells expressing intense nuclear RB-IR staining and the number of thymidine-labeled cells that had differentiated into functional hippocampal neurons. A substantial decrease in RB-IR during differentiation was also observed and occurred prior to the expression of NSE. The possibility that the loss of RB may be necessary for neuronal differentiation to proceed is discussed.