The effects of the somatostatin analog octreotide on angiogenesis in vitro
The effects of the somatostatin analog octreotide on angiogenesis in vitro
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DOI：
10.1016/s0026-0495(96)90080-3
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发表时间：
1996-08-01
影响因子：
9.8
通讯作者：
DelTacca, M
中科院分区：
文献类型：
--
作者：
Danesi, R;DelTacca, M
本研究考察了生长抑素类似物奥曲肽对人HUV-EC-C内皮细胞和在纤连蛋白包被培养皿上培养或包含在纤维蛋白凝胶中培养的大鼠主动脉外植体的血管细胞生长的体外抗血管生成作用，总10(-9) mol/L奥曲肽使HUV-EC-C细胞对H-3-胸苷的平均摄取量与对照组相比降低了37%。控制。通过四唑生物还原和图像分析分别测定，与对照组相比，10(-8) mol/L浓度的奥曲肽可抑制纤连蛋白上生长的内皮细胞和平滑肌细胞的增殖32.6%，使纤维蛋白中主动脉环外膜细胞的出芽减少33.2%。这些结果证明奥曲肽是体外血管细胞增殖的有效抑制剂。版权所有 (C) 1996 W.B.桑德斯公司。
This study examined the in vitro antiangiogenic effects of the somatostatin analog octreotide on the growth of human HUV-EC-C endothelial cells and vascular cells from explants of rat aorta cultured on fibronectin coated dishes or included in fibrin gel, A total 10(-9) mol/L octreotide reduced the mean uptake of H-3-thymidine by HUV-EC-C cells by 37% compared with controls. The 10(-8) mol/L concentration of octreotide inhibited the proliferation of endothelial and smooth muscle cells growing on fibronectin by 32.6% and reduced the sprouting of cells from the adventitia of aortic rings in fibrin by 33.2% compared with controls, as measured by tetrazolium bioreduction and image analysis, respectively. These results demonstrate that octreotide is an effective inhibitor of vascular cell proliferation in vitro. Copyright (C) 1996 by W.B. Saunders Company.