IDENTIFICATION OF THE ENVELOPE V3 LOOP AS THE PRIMARY DETERMINANT OF CELL TROPISM IN HIV-1
IDENTIFICATION OF THE ENVELOPE V3 LOOP AS THE PRIMARY DETERMINANT OF CELL TROPISM IN HIV-1
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DOI：
10.1126/science.1905842
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发表时间：
1991-07-05
期刊：
影响因子：
56.9
通讯作者：
CULLEN, BR
中科院分区：
文献类型：
--
作者：
HWANG, SS;BOYLE, TJ;CULLEN, BR
单核细胞-巨噬细胞谱系的细胞是体内人免疫缺陷病毒-1（HIV-1）感染的靶。然而，许多有效感染转化T细胞系的HIV-1实验室菌株在巨噬细胞中复制较差。来自HIV-1的巨噬细胞嗜性BaL分离物的20个氨基酸序列足以赋予巨噬细胞对HTLV-IIIB（一种T细胞系嗜性分离物）的嗜性。这个小的序列元件位于V3环中，V3环是HIV-1的主要中和决定簇。因此，V3环不仅充当宿主免疫应答的靶标，而且在确定HIV-1组织嗜性中也是关键的。
Cells of the monocyte-macrophage lineage are targets for human immunodeficiency virus-1 (HIV-1) infection in vivo. However, many laboratory strains of HIV-l that efficiently infect transformed T cell lines replicate poorly in macrophages. A 20-amino acid sequence from the macrophage-tropic BaL isolate of HIV-1 was sufficient to confer macrophage tropism on HTLV-IIIB, a T cell line-tropic isolate. This small sequence element is in the V3 loop, the envelope domain that is the principal neutralizing determinant of HIV-1. Thus, the V3 loop not only serves as a target of the host immune response but is also pivotal in determining HIV-l tissue tropism.