In Situ Derivatization and Quantification of Seven Carbonyls in Cigarette Mainstream Smoke
In Situ Derivatization and Quantification of Seven Carbonyls in Cigarette Mainstream Smoke
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DOI：
10.1021/acs.chemrestox.5b00474
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发表时间：
2016-01-01
影响因子：
4.1
通讯作者：
Watson, Clifford H.
中科院分区：
文献类型：
--
作者：
Ding, Yan S.;Yan, Xizheng;Watson, Clifford H.
羰基化合物，特别是醛类，是一组存在于烟草烟雾中的有害挥发性有机化合物。七种羰基化合物被列入FDA关于烟草或烟草烟雾的有害和潜在有害成分清单。羰基具有反应性官能团，因此在卷烟烟气中定量测量具有挑战性。传统的测量烟雾中羰基化合物的方法包括填充溶剂的撞击采样器捕集和衍生化。这一过程是劳动密集型的，并产生大量的危险废物。我们开发了一种新的方法，有效地衍生和捕获羰基从主流烟雾原位剑桥过滤垫。从垫中提取衍生的羰基化合物，随后通过超高压液相色谱-串联质谱法进行定量。新方法已被验证并应用于研究和商业卷烟。研究香烟的羰基产量与其他已发表文献数据的羰基产量相当。通过方便的烟雾收集装置，4分钟的样品分析时间，以及低或亚微克的检测限，这种新方法不仅简化和加速了主流烟雾中一类重要化学成分的检测，而且还减少了反应损失，并提供了更准确的评估烟雾中的羰基水平。出色的准确度（平均98%）和精确度（研究卷烟的平均相对标准偏差为14%）确保了这种新方法具有足够的保真度来表征传统燃烧的烟草产品，并有可能应用于新产品或新兴产品。
Carbonyls, especially aldehydes, are a group of harmful volatile organic compounds that are found in tobacco smoke. Seven carbonyls are listed on the FDA's harmful and potential harmful constituents list for tobacco or tobacco smoke. Carbonyls have reactive functional groups and thus are challenging to quantitatively measure in cigarette smoke. The traditional method of measuring carbonyls in smoke involves solvent-filled impinger trapping and derivatization. This procedure is labor-intensive and generates significant volumes of hazardous waste. We have developed a new method to efficiently derivatize and trap carbonyls from mainstream smoke in situ on Cambridge filter pads. The derivatized carbonyls are extracted from the pads and subsequently quantified by ultra-high-pressure liquid chromatography coupled with tandem mass spectrometry. The new method has been validated and applied to research and commercial cigarettes. Carbonyl yields from research cigarettes are comparable to those from other published literature data. With a convenient smoke collection apparatus, a 4 min sample analysis time, and a low- or submicrogram detection limit, this new method not only simplifies and speeds the detection of an important class of chemical constituents in mainstream smoke but also reduces reactive losses and provides a more accurate assessment of carbonyl levels in smoke. Excellent accuracy (average 98%) and precision (14% average relative standard deviation in research cigarettes) ensure this new methods sufficient fidelity to characterize conventional combusted tobacco products, with potential application toward new or emerging products.