Bioactive Metabolites from the Deep-Sea-Derived FungusDiaporthe longicollaFS429
Bioactive Metabolites from the Deep-Sea-Derived FungusDiaporthe longicollaFS429
复制标题
深海真菌 Diaporthe longicolla FS429 的生物活性代谢物
DOI：
10.3390/md18080381
复制
发表时间：
2020-08-01
期刊：
影响因子：
5.4
通讯作者：
Zhang, Weimin
中科院分区：
文献类型：
--
作者：
Liu, Zhaoming;Chen, Yuchan;Zhang, Weimin
对深海真菌Diaporthe Longicolla FS429的甲醇提取物进行了化学研究，分离得到了两个新的二萜类长二酸A和B(1和2)，两个新的多酮(3和4)，两个新的细胞松弛素类似物长春花素A和B(6和8)和三个已知的类似物5，7，9。通过综合光谱分析确定了它们的结构，并通过实验和量子化学计算的ECD光谱的比较确定了它们的绝对构型。化合物7的结构首次通过X-射线衍射法确证。在生物活性测定中，化合物8对SF-268细胞有抑制增殖作用，IC50值为16.44μM。在50μM浓度下，化合物1和8对结核分枝杆菌蛋白酪氨酸磷酸酶B的抑制活性分别为35.4%和53.5%。
The chemical investigation of a methanol extract of the deep-sea-derived fungus Diaporthe longicolla FS429 led to the isolation of two novel diterpenoids longidiacids A and B (1 and 2), two new polyketides (3 and 4), two new cytochalasin analogues longichalasins A and B (6 and 8) and three known analogues 5, 7, 9. Their structures were elucidated through comprehensive spectroscopic analysis, while the absolute configurations were established by the comparison of the experimental and quantum chemical calculated ECD spectra. The structure of compound 7 was confirmed through X-ray diffraction for the first time. In the bioassays compound 8 exhibited antiproliferative effects against SF-268, with an IC50 value of 16.44 μM. Moreover, compounds 1 and 8 were detected to inhibit 35.4% and 53.5% of enzyme activity of Mycobacterium tuberculosis protein tyrosine phosphatase B (MptpB) at a concentration of 50 μM.