Antimicrobial Properties of Light-activated Polyurethane Containing Indocyanine Green
Antimicrobial Properties of Light-activated Polyurethane Containing Indocyanine Green
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DOI：
10.1177/0885328209352701
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发表时间：
2011-01-01
影响因子：
2.9
通讯作者：
Prokopovich, Polina
中科院分区：
文献类型：
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作者：
Perni, Stefano;Pratten, Jonathan;Prokopovich, Polina
本研究的目的是生产新型的抗菌聚合物含有光活化抗菌剂吲哚菁绿色（ICG）。通过在含有水和ICG的丙酮中溶胀聚氨酯，然后蒸发溶剂来制备新型材料。ICG的摄取依赖于丙酮与水的比例。只有在99份丙酮对1份水的比例下，样品才有任何实质性的着色。当暴露于近红外光谱（808 nm）的激光时，含多聚赖氨酸的ICG对革兰氏阳性菌表现出抗菌活性;暴露15分钟后，对耐甲氧西林金黄色葡萄球菌（MRSA）和表皮葡萄球菌的抗菌活性降低了2 log（10），对应于能量密度为31.83 J/cm（2）的能量剂量为31.83 J。在相同条件下，革兰氏阴性菌（大肠杆菌和铜绿假单胞菌）似乎不太敏感，活菌计数减少0.5 log（10）。还研究了所得材料的一些物理性质，发现弹性（杨氏模量）降低了约60%。此外，当拉伸含ICG的聚合物时，断裂点出现在伸长率为初始值的6.7倍时，而不含ICG的聚氨酯样品在7倍伸长率后不断裂。水滴的接触角表明，含ICG的聚合物比未处理的聚氨酯更疏水。本研究结果表明，ICG可以嵌入聚氨酯中，制成在近红外光照射下可以发挥杀菌作用的材料，特别是对MRSA和S.表皮这样的材料可用于制备静脉内导管，静脉内导管通常被这样的生物体定殖。
The aim of this study was to produce novel antimicrobial polymers containing the light-activated antimicrobial agent indocyanine green (ICG). The novel materials were prepared by swelling polyurethane in acetone containing water and ICG, followed by solvent evaporation. The uptake of ICG was dependent upon the ratio of acetone to water. Only at a ratio of 99 parts acetone to 1 part water was there any substantial colouration of the samples. When exposed to laser light from the near infrared spectrum (808 nm), polyurethane-containing ICG exhibited antimicrobial activity against Gram-positive bacteria; a 2 log(10) reduction was achieved against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis after 15 min exposure, corresponding to an energy dose of 31.83 J delivered at an energy density of 31.83 J/cm(2). Under the same conditions, Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) appeared to be less susceptible, the viable count being reduced by 0.5 log(10). Some of the physical properties of the resulting material were also investigated and it was found that the elasticity (Young's modulus) was reduced by approximately 60%. Furthermore, when the ICG-containing polymer was stretched, the breaking point occurred when the elongation was 6.7 times the initial value, while ICG-free polyurethane samples did not break following a 7-fold elongation. The contact angles for water droplets revealed that the ICG-containing polymer was more hydrophobic than untreated polyurethane.The results of this study show that ICG can be embedded in polyurethane to produce materials which when irradiated with near-infrared light can exert a bactericidal effect particularly against MRSA and S. epidermidis. Such materials may be useful for preparing intravenous catheters, which are often colonized by such organisms.