MOLECULAR-CLONING AND CHARACTERIZATION OF A RAT ADENOSINE-A1-RECEPTOR THAT IS WIDELY EXPRESSED IN BRAIN AND SPINAL-CORD
MOLECULAR-CLONING AND CHARACTERIZATION OF A RAT ADENOSINE-A1-RECEPTOR THAT IS WIDELY EXPRESSED IN BRAIN AND SPINAL-CORD
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DOI：
10.1210/mend-5-8-1037
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发表时间：
1991-08-01
影响因子：
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通讯作者：
RIVKEES, SA
中科院分区：
文献类型：
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作者：
REPPERT, SM;WEAVER, DR;RIVKEES, SA
用聚合酶链反应产生的探针从大鼠脑cDNA文库中克隆了A1-腺苷受体。 该cDNA编码327个氨基酸的蛋白质，其与最近克隆的狗A1-腺苷受体（RDC 7）具有91%的同一性。 大鼠cDNA在COS-6 M和NIH 3 T3细胞中的表达导致与腺苷酸环化酶抑制偶联的A1-腺苷受体的配体结合和功能活性特征。 通过北方印迹分析检测A1-腺苷受体mRNA的分布，表明其在脑、脊髓、睾丸和白色脂肪组织中高度表达。 原位杂交研究揭示了中枢神经系统中广泛的杂交模式，在大脑皮层、海马、小脑、丘脑、脑干和脊髓中具有高水平。 因此，克隆的A1-腺苷受体可能介导腺苷在神经和内分泌系统中的许多调节作用。
An A1-adenosine receptor has been cloned from a rat brain cDNA library using a probe generated by the polymerase chain reaction. The cDNA encodes a protein of 327 amino acids which is 91% identical to a recently cloned dog A1-adenosine receptor (RDC7). Expression of the rat cDNA in COS-6M and NlH 3T3 cells resulted in ligand binding and functional activity characteristics of an A1-adenosine receptor that is coupled to inhibition of adenylyl cyclase. Examination of the distribution of A1-adenosine receptor mRNA by Northern blot analysis showed that it is highly expressed in brain, spinal cord, testis, and white adipose tissue. In situ hybridization studies revealed an extensive hybridization pattern in the central nervous system, with high levels in cerebral cortex, hippocampus, cerebellum, thalamus, brainstem, and spinal cord. The cloned A1-adenosine receptor may thus mediate many of the modulatory actions of adenosine in neural and endocrine systems.