Effects of blue light on gametophyte development ofLaminaria japonica (Laminariales, Phaeophyta)
Effects of blue light on gametophyte development ofLaminaria japonica (Laminariales, Phaeophyta)
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DOI：
10.1007/bf02847155
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发表时间：
2005-08
影响因子：
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通讯作者：
Cuijuan Shi;Kataoka Hironao;Duan Delin
中科院分区：
文献类型：
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作者：
Cuijuan Shi;Kataoka Hironao;Duan Delin
叠层附生植物的生长发育受光照的影响较大。采用发光二极管（LED）作为蓝光和红光光源，分析了光照对日本火龙花配子体发育的影响。配子体来源于2003年4月、5月、7月和2004年9月采集的游动孢子。结果表明，增加蓝光和红光照射强度均能促进配子体的生长，其中蓝光明显强于红光。配子体的受精在很大程度上依赖于光照，只有充足的光照才能达到繁殖效果。此外，我们注意到不同时间收集的游动孢子发育的配子体对光的响应有显著差异。对于4月份释放的游动孢子，在BL1（73.90 μmol光子/m·s）条件下，单细胞雌配子体和多细胞雄配子体分别产生卵子和精子，并向孢子体发育。而在5、7、9月份发育的配子体则是多细胞的，不形成卵原体和卵原体。认为在实验室培养条件下，绒条菊成熟阶段可能影响配子体对BL的反应。因此，隐色素或嗜光素样的BL光感受器可能参与了BL诱导的植物发育过程。
Laminariagametophyte was greatly influenced by light in its growth and development. Using light-emitting diodes (LED) as blue and red light sources, we analyzed the light effect on gametophytes development ofLaminaria japonicaAresch. The gametophytes were obtained from zoospores collected in April, May, July, 2003 and September, 2004. We found that the growth of gametophytes was stimulated by increasing intensity of blue light (BL) and red light (RL) illumination, of which BL was obviously stronger than that of RL. The fertilization of gametophytes depended largely on BL, and only sufficient BL illumination could take the reproductive effect. In addition, we noticed that there was a significant difference in light responses for gametophytes developed from zoospore collected in different times. For zoospores released in April, under BL1(73.90 μmol photons/m·s), the unicellular female gametophytes and multi-cellular male gametophytes produced eggs and sperms respectively, and further developed towards sporophytes. However, for gametophytes developed in May, July or September, they became multi-cellular and never formed oogonia or antheridia. It is believed that theLaminariasporangium maturation stage could affect the gametophytes reaction to BL under laboratory culture conditions. Therefore, cryptochrome- or phototropin-like BL photoreceptors is probably involved in BL-induced development ofLaminariagametophytes.