Proteomic analysis of cellular protein alterations using a hepatitis B virus‐producing cellular model
Proteomic analysis of cellular protein alterations using a hepatitis B virus‐producing cellular model
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DOI：
10.1002/pmic.200700849
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发表时间：
2008-05
期刊：
影响因子：
3.4
通讯作者：
A. Tong;Lihong Wu;Q. Lin;Q. Lau;Xia Zhao;Jiong Li;Ping Chen;Li-juan Chen;Hong Tang;
中科院分区：
文献类型：
--
作者：
A. Tong;Lihong Wu;Q. Lin;Q. Lau;Xia Zhao;Jiong Li;Ping Chen;Li-juan Chen;Hong Tang;
乙型肝炎病毒（HBV）是导致急性和慢性肝病以及肝细胞癌（HCC）发展的主要病因之一。为了确定 HBV 复制对宿主细胞蛋白质表达的影响，我们利用 2-DE 和 MS/MS 分析来比较和鉴定产生 HBV 的细胞系 HepG2.2.15 与其亲本细胞系 HepG2 之间的差异表达蛋白质。在两个细胞系之间被鉴定为差异表达（±超过两倍，p <0.05）的 66 个点中，MS/MS 分析阳性鉴定了 62 个点（对应于 61 个独特的蛋白质）。通过聚类和代谢/信号通路分析，这些蛋白质可以清楚地分为三大类：参与视黄醇代谢途径的蛋白质、钙离子结合蛋白和与蛋白质降解途径相关的蛋白质。其他已鉴定的蛋白质包括在多种生物过程中发挥作用的蛋白质，例如信号转导、免疫调节、分子伴侣、电子传递/氧化还原调节、细胞增殖/分化和 mRNA 剪接。总之，我们分析了 HepG2.2.15 和 HepG2 细胞之间的蛋白质组变化。本研究中鉴定的蛋白质将有助于揭示 HBV 与宿主细胞相互作用和 HCC 发展的潜在机制。这项研究也可以为抗病毒研究提供一些有用的线索。
Hepatitis B virus (HBV) is one of the major etiological factors responsible for acute and chronic liver disease and for the development of hepatocellular carcinoma (HCC). To determine the effects of HBV replication on host cell‐protein expression, we utilized 2‐DE and MS/MS analysis to compare and identify differentially expressed proteins between an HBV‐producing cell line HepG2.2.15 and its parental cell line HepG2. Of the 66 spots identified as differentially expressed (± over twofold, p <0.05) between the two cell lines, 62 spots (corresponding to 61 unique proteins) were positively identified by MS/MS analysis. These proteins could be clearly divided into three major groups by cluster and metabolic/signaling pathway analysis: proteins involved in retinol metabolism pathway, calcium ion‐binding proteins, and proteins associated with protein degradation pathways. Other proteins identified include those that function in diverse biological processes such as signal transduction, immune regulation, molecular chaperone, electron transport/redox regulation, cell proliferation/differentiation, and mRNA splicing. In summary, we profiled proteome alterations between HepG2.2.15 and HepG2 cells. The proteins identified in this study would be useful in revealing the mechanisms underlying HBV‐host cell interactions and the development of HCC. This study can also provide some useful clues for antiviral research.