Comparison of calcium and hydroxyl ion release ability and in vivo apatite-forming ability of three bioceramic-containing root canal sealers
Comparison of calcium and hydroxyl ion release ability and in vivo apatite-forming ability of three bioceramic-containing root canal sealers
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DOI：
10.1007/s00784-021-04118-w
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发表时间：
2021-08-16
影响因子：
3.4
通讯作者：
Noiri, Yuichiro
中科院分区：
文献类型：
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作者：
Belal, Razi Saifullah Ibn;Edanami, Naoki;Noiri, Yuichiro
目的生物陶瓷根管封闭剂通过钙离子和氢氧根离子的释放和表面磷灰石的形成促进根尖周愈合。本研究旨在比较三种含生物陶瓷的根管密封剂：EndoSequence BC密封剂（Endo-BC）， MTA Fillapex （MTA- f）和Nishika根管密封剂BG （N-BG）的Ca2+和OH-释放和体内磷灰石形成。材料和方法将聚四氟乙烯管装入密封剂，在蒸馏水中浸泡6和12小时，分别浸泡1、7、14和28天，测量Ca2+和OH-的释放。另外，在大鼠背部植入装有密封剂的管子28天，用电子探针微量分析仪分析体内磷灰石的形成。结果Endo-BC在6、12 h和1 d释放的Ca2+明显高于其他封闭剂。在第14和28天，N-BG的Ca2+释放量明显低于Endo-BC和MTA-F。在整个实验过程中，除第1天外，Endo-BC的OH-释放量显著高于其他封口剂。6 h和7 d时，N-BG的OH-释放量低于MTA-F。只有Endo-BC植入物表现出磷灰石样的富钙、富磷、富氧和富碳球晶和磷灰石层状的富钙、富磷，但不透射线的无元素表面区域。结论Ca2+和OH-释放顺序如下：Endo-BC > MTA-F > N-BG。只有Endo-BC能在体内形成磷灰石。
Objective Bioceramic-containing root canal sealers promote periapical healing via Ca2+ and OH- release and apatite formation on the surface. This study aimed to compare Ca2+ and OH- release and in vivo apatite formation of three bioceramic-containing root canal sealers: EndoSequence BC sealer (Endo-BC), MTA Fillapex (MTA-F), and Nishika Canal Sealer BG (N-BG). Materials and methods Polytetrafluoroethylene tubes filled with sealers were immersed in distilled water for 6 and 12 h and for 1, 7, 14, and 28 days to measure Ca2+ and OH- release. Additionally, tubes filled with sealers were implanted in the backs of rats for 28 days, and in vivo apatite formation was analyzed using an electron probe microanalyzer. Results Endo-BC released significantly more Ca2+ than the other sealers at 6 and 12 h and 1 day. Ca2+ release was significantly lower from N-BG than from Endo-BC and MTA-F at 14 and 28 days. OH- release was significantly higher from Endo-BC than from the other sealers throughout the experiment, except at 1 day. OH- release was lower from N-BG than from MTA-F at 6 h and 7 days. Only Endo-BC implants exhibited apatite-like calcium-, phosphorus-, oxygen-, and carbon-rich spherulites and apatite layer-like calcium- and phosphorus-rich, but radiopaque element-free, surface regions. Conclusions Ca2+ and OH- release is ranked as follows: Endo-BC > MTA-F > N-BG. Only Endo-BC demonstrated in vivo apatite formation.