Signed, sealed, and delivered: RNA localization and translation at centrosomes.
Signed, sealed, and delivered: RNA localization and translation at centrosomes.
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DOI：
10.1091/mbc.e21-03-0128
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发表时间：
2022-05-01
影响因子：
3.3
通讯作者：
Lerit DA
中科院分区：
文献类型：
--
作者：
Lerit DA
蛋白质定位是细胞功能和专门活动（如迁移或增殖）所固有的。许多局部蛋白质富集在确定的细胞器中，形成由相互作用的蛋白质组装体进一步指定的功能活性的子域。中心体是一个研究得很好的细胞器，它显示了蛋白质组成的动态功能变化。中心体是微管组织中心，具有多种细胞功能，主要由中心粒周围物质的组成来定义，中心粒周围物质是围绕中心粒对组织的有序蛋白质基质。mRNA也定位于中心粒周围物质。虽然几十年前就在中心体发现了RNA，但特定RNA转录本的特征及其对中心体生物学的功能贡献在很大程度上仍未得到研究。虽然定位于中心体的RNA的鉴定随着高通量筛选方法的发展而加速，但这一发现仍然超过了功能表征。最近的工作表明定位于中心体的RNA具有生物学意义，并进一步暗示中心体是局部蛋白质合成的位点。不同的RNA定位和翻译活动可能有助于细胞内中心体功能的多样性。
Protein localization is intrinsic to cellular function and specialized activities, such as migration or proliferation. Many localized proteins enrich at defined organelles, forming subdomains of functional activity further specified by interacting protein assemblies. One well-studied organelle showing dynamic, functional changes in protein composition is the centrosome. Centrosomes are microtubule-organizing centers with diverse cellular functions largely defined by the composition of the pericentriolar material, an ordered matrix of proteins organized around a central pair of centrioles. Also localizing to the pericentriolar material are mRNAs. Although RNA was identified at centrosomes decades ago, the characterization of specific RNA transcripts and their functional contributions to centrosome biology remained largely unstudied. While the identification of RNA localized to centrosomes accelerated with the development of high-throughput screening methods, this discovery still outpaces functional characterization. Recent work indicates RNA localized to centrosomes is biologically significant and further implicates centrosomes as sites for local protein synthesis. Distinct RNA localization and translational activities likely contribute to the diversity of centrosome functions within cells.
影响因子：
7.8
作者：
Karsenti, E;Kobayashi, S;Mitchison, T;Kirschner, M
通讯作者：
Kirschner, M