A Validated High-performance Liquid Chromatograhy Method for Estimation of Ferulic Acid in Asafoetida and Polyherbal Preparation.
A Validated High-performance Liquid Chromatograhy Method for Estimation of Ferulic Acid in Asafoetida and Polyherbal Preparation.
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DOI：
10.4103/0250-474x.119826
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发表时间：
2013-07
影响因子：
0.5
通讯作者：
Kadam VJ
中科院分区：
文献类型：
--
作者：
Kareparamban JA;Nikam PH;Jadhav AP;Kadam VJ
建立了高效液相色谱法测定阿魏酸含量的方法。色谱柱为HiQSil ODS C-18，流动相为乙腈：10%醋酸（20:80 v/v）。根据国际协调会议准则对准确度、精密度、线性度、检出限、定量限和特异性等参数进行了验证；并且被认为是可靠的。线性回归分析表明，在200 ~ 7000 ng/ml范围内，峰面积与浓度具有良好的相关性，相关系数r2=0.996。所建立的方法可用于菝葜中药制剂的标准化。
A high-performance liquid chromatography method was developed for the estimation of ferulic acid from asafoetida and a polyherbal preparation. The separation was carried out on HiQSil ODS C-18 column with a mobile phase of acetonitrile: 10% acetic acid (20:80 v/v). The developed method was validated as per International Conference of Harmonization guidelines for various parameters such as accuracy, precision, linearity, limit of detection, limit of quantification and specificity; and found to be reliable. Linear regression analysis showed a good corelation between peak area and concentration with a corelation coefficient r2=0.996 in the range 200-7000 ng/ml. The developed method can be utilized for standardization of herbal formulation comprising asafoetida.