Label-Free Immunosensing for α-Fetoprotein in Human Plasma using Surface Plasmon Resonance
Label-Free Immunosensing for α-Fetoprotein in Human Plasma using Surface Plasmon Resonance
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DOI：
10.7567/ssdm.2007.d-9-4
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发表时间：
2007-06
期刊：
影响因子：
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通讯作者：
Keiko Kawano;Y. Teramura;Motohiro Oda;Takaaki Suzuki;H. Kotera;H. Iwata
中科院分区：
文献类型：
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作者：
Keiko Kawano;Y. Teramura;Motohiro Oda;Takaaki Suzuki;H. Kotera;H. Iwata
在本研究中，我们试图开发一种基于表面等离子体共振（SPR）的免疫测定传感器，在纳克水平上检测人血浆中α-胎蛋白（AFP），用于肝细胞肿瘤的临床诊断。采用三（乙二醇）（TEG）和端羧基六（乙二醇）（HEG）自组装单层（SAM）表面来抑制等离子体组分在传感器表面的非特异性吸附。采用夹心式免疫分析法检测甲胎蛋白（AFP），采用一抗和二抗两种抗体。利用针对第二抗体的抗体（多克隆）进一步增强SPR信号移位。该方法具有SPR信号高度强化的特点，能以较高的信噪比方便地检测出毫微克水平（ng/ml）的AFP，为临床诊断提供了必要条件。期望我们基于spr的免疫分析方法也可以适用于检测人体血液中低浓度存在的其他几种肿瘤标志物。
In this study, we attempted to develop a surface plasmon resonance (SPR)-based immunoassay sensor to detect α-fetoprotein (AFP) in human plasma at the nanogram level, as is required for clinical diagnosis of hepatocellular tumors. A self-assembled monolayer (SAM) surface of tri(ethylene glycol) (TEG) and carboxyl group-terminated hexa(ethylene glycol) (HEG) was employed to suppress the nonspecific adsorption of plasma components onto the sensor surface. AFP was detected by a sandwich-type immunoassay using two kinds of antibodies, primary and secondary, in this system. The SPR signal shift was further enhanced by applying an antibody (polyclonal) against the second antibody. With this method, the SPR signals were highly intensified, and so nanogram levels (ng/ml) of AFP could be easily detected with a high signal/noise ratio, as is necessary for clinical diagnosis. It is expected that our SPR-based immunoassay method can also be applicable to the detection of several other tumor markers that are present in low concentrations in human blood.