Using Matrix Solid-Phase Microextraction (Matrix-SPME) to Estimate Bioavailability of DDTs in Soil to Both Earthworm and Vegetables
Using Matrix Solid-Phase Microextraction (Matrix-SPME) to Estimate Bioavailability of DDTs in Soil to Both Earthworm and Vegetables
复制标题
使用基质固相微萃取 (Matrix-SPME) 估算土壤中滴滴涕对蚯蚓和蔬菜的生物利用度
DOI：
10.1007/s00244-009-9329-4
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发表时间：
2010-01-01
影响因子：
4
通讯作者：
Yu, Yunlong
中科院分区：
文献类型：
--
作者：
Fang, Hua;Chu, Xiaoqiang;Yu, Yunlong
本研究旨在寻找一种合适的方法来评估土壤中DDTs对蔬菜和蔬菜的生物有效性。四种化学方法-正己烷索氏萃取、正丁醇搅拌萃取、水搅拌萃取和基质固相微萃取（基质-SPME）-用于评估1，1-二氯-2，2-双对氯苯基乙烯（p，p '-DDE），1，1，1-三氯-2-对氯苯基邻氯苯基乙烷（o，p'-DDT），1，1-dichloro-2，2-bis对氯苯基乙烷（p，p '-DDD）和1，1，1-三氯-2，2-双对氯苯基乙烷（p，p '-DDT）在土壤中的含量及其在油菜、白菜、水稻和水稻中的吸收量。spp.），和科尔（Brassica napus L.）。这些结果表明，土壤中DDTS的可提取性和生物有效性随着老化时间的延长而降低。相关性分析表明，正丁醇萃取法和12 h基质固相微萃取法可用于评价DDTs对油菜的生物有效性，索氏提取法、正丁醇萃取法和12 h基质固相微萃取法可用于预测DDTs对白菜和科尔的生物有效性。基质固相微萃取法是一种无溶剂、快速、可忽略的方法，与索氏提取法、正丁醇提取法和水提取法相比，基质固相微萃取法能够更好地预测DDTs在蔬菜和蔬菜中的生物有效性。
This study was conducted to find an appropriate approach for the assessment of bioavailability of DDTs in soil to both earthworm and vegetables. Four chemical approaches-Soxhlet extraction with n-hexane, n-butanol agitation extraction, water agitation extraction, and matrix solid-phase microextraction (matrix-SPME)-were used to assess the relationships between the extractability of 1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene (p,p'-DDE), 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) ethane (o,p'-DDT), 1,1-dichloro-2,2-bis(p-chlorophenyl) ethane (p,p'-DDD), and 1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane (p,p'-DDT) in soil and their amounts uptaken by the earthworm (Eisenia foetida), Chinese cabbage (Brassica campestris L. spp.), and cole (Brassica napus L.). These results indicated that the extractability and bioavailability of DDTs in soil decreased with time of aging. Correlation analysis showed that n-butanol extraction or 12-h matrix-SPME could be used to assess the bioavailability of DDTs to the earthworm, and Soxhlet extraction, n-butanol extraction, or 12-h matrix-SPME could be used to predict the bioavailability of DDTs to both Chinese cabbage and cole. As a solventless, time-efficient, and negligible-depletion technique, it could be concluded that matrix-SPME is a better approach to predict the bioavailability of DDTs to both the earthworm and vegetables, compared with Soxhlet extraction, n-butanol extraction, and water extraction.