A metabolomic study on early detection of steroid-induced avascular necrosis of the femoral head.
A metabolomic study on early detection of steroid-induced avascular necrosis of the femoral head.
复制标题
早期检测类固醇引起的股骨头缺血性坏死的代谢组学研究
DOI：
10.18632/oncotarget.24150
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发表时间：
2018-01-30
期刊：
影响因子：
--
通讯作者：
Liang Q
中科院分区：
文献类型：
--
作者：
Ren X;Fan W;Shao Z;Chen K;Yu X;Liang Q
激素性股骨头缺血性坏死（SANFH）病情发展不可逆，给患者带来严重后果，因此早期准确诊断该病具有重要意义。本研究旨在探讨SANFH的代谢变化，为早期发现SANFH提供依据。本研究设计了两个阶段，即发现和验证。除生化指标异常和意外死亡外，30只健康成年日本白色家兔用于筛选发现实验中的潜在代谢物，13只用于验证实验。采用磁共振成像和透射电镜对股骨头进行评估。采用超高效液相色谱-串联质谱法（UHPLC-MS/MS）对血清样品进行代谢组学分析。代谢组学聚类分析使我们能够区分1周内未注射糖皮质激素和注射糖皮质激素的家兔，即使在行为或影像学诊断上没有明显异常症状。糖皮质激素注射后1、2、3周，差异代谢产物主要为磷脂类物质。6周的验证实验结果表明，这些差异代谢产物在晚期进展中与早期进展中的趋势一致。6周后病理学检查证实SANFH损伤。因此，血清代谢物谱的发现与SANFH的进展有关，并提供了早期检测SANFH的可能性。
The early and accurate diagnosis of steroid-induced avascular necrosis of the femoral head (SANFH) is appealing considering its irreversible progression and serious consequence for the patients. The purpose of this study was to investigate the metabolic change of SANFH for its early detection. Two stages were designed in this study, namely discovery and verification. Except the biochemical index anomaly and the accidental death, 30 adult healthy adult Japanese white rabbits were used for screening out the potential metabolites in discovery experiment and 13 rabbits were used in verification experiment. The femoral heads were assessed with magnetic resonance imaging and transmission electron microscopy. The metabolomic profiling of serum samples were analysis by UHPLC-MS/MS. Metabolomic cluster analysis enable us to differentiate the rabbits without and with injection of the glucocorticoid in 1 week even when there is no obvious abnormal symptom in behaviors or imaging diagnosis. The majority of differential metabolites were identified as phospholipids which were observed significant change after injection of glucocorticoid in 1, 2, 3 weeks. And the results obtained in verification experiment of 6 weeks showed that these differential metabolites exhibited consistent trends in late progression with that in early-stage. At the end of 6 weeks the damage of SANFH could be verified by pathological imaging. Therefore the finding of serum metabolite profile links to the progression of SANFH and provides the potential of early detection of SANFH.