Selection of High-Producing Clones Using FACS for CHO Cell Line Development
Selection of High-Producing Clones Using FACS for CHO Cell Line Development
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DOI：
10.1007/978-1-4939-6972-2_9
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发表时间：
2017-01-01
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影响因子：
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通讯作者：
Kelly, Paul S.
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文献类型：
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作者：
Gallagher, Clair;Kelly, Paul S.
细胞系开发旨在产生和选择具有所需特征的克隆。生物制药细胞选择的最重要参数之一是细胞比生产率（Qp）或每天每个细胞产生的产物的量。流式细胞仪是一种功能强大的高通量技术，有助于从异质群体中分离单个细胞克隆并进行多参数表征。在这里，我们描述了一种基于流式细胞术的方法，用于高产量CHO细胞克隆的切片。
Cell line development aims to generate and select clones with desirable characteristics. One of the most important parameters for biopharmaceutical cell selection is cell-specific productivity (Qp) or the quantity of product produced per cell per day. Fluorescence-activated cell sorting (FACS) is a powerful, high-throughput technique that facilitates multiparametric characterization and isolation of individual cell clones from heterogeneous populations. Here, we describe a FACS-based method for section of high-producing CHO cell clones.