Role of oxidative phosphorylation and ATP release in mediating birth-related pulmonary vasodilation in fetal lambs
Role of oxidative phosphorylation and ATP release in mediating birth-related pulmonary vasodilation in fetal lambs
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DOI：
10.1152/ajpheart.00245.2002
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发表时间：
2002-10-01
影响因子：
4.8
通讯作者：
Mattei, J
中科院分区：
文献类型：
--
作者：
Konduri, GG;Mattei, J
我们研究了一种假说，即与出生相关的肺血管舒张在一定程度上是由氧化磷酸化和ATP释放的增加介导的，这是出生时对氧气暴露的反应。在胎羊中进行了研究，以评估氧气、单独肺扩张或肺扩张伴氧和剪切应力对胎儿肺血流和阻力以及肺动脉血浆ATP水平的独立影响。将羔羊分为三组：对照组或预处理2,4-二硝基苯酚或抗霉素- a，氧化磷酸化抑制剂，评估每种干预措施的效果。单独暴露于氧气或肺扩张与血浆ATP水平和肺血流量的增加以及肺血管阻力的降低有关。血浆ATP水平在肺扩张期间没有变化。2,4-二硝基苯酚和抗霉素- a可减弱肺血管舒张剂对氧气的反应，但不能减弱单独对肺扩张的反应。在氧气暴露期间氧化磷酸化和ATP释放的增加可能有助于胎儿羔羊的出生相关肺血管舒张。
We investigated the hypothesis that birth-related pulmonary vasodilation is mediated in part by an increase in oxidative phosphorylation and ATP release in response to oxygen exposure at birth. Studies were done in fetal lambs to evaluate the independent effects of oxygen, lung distension alone, or lung distension accompanied by oxygenation and shear stress on fetal pulmonary blood flow and resistance and plasma ATP levels in the pulmonary artery. The effect of each intervention was evaluated in lambs assigned to one of three groups: control or pretreatment with 2,4-dinitrophenol or antimycin-A, inhibitors of oxidative phosphorylation. Exposure to oxygen alone or with lung distension was associated with increases in plasma ATP levels and pulmonary blood flow and a decrease in pulmonary vascular resistance. Plasma ATP levels did not change during lung distension alone. 2,4-Dinitrophenol and antimycin-A attenuated the pulmonary vasodilator response to oxygen but did not attenuate the response to lung distension alone. An increase in oxidative phosphorylation and ATP release during oxygen exposure may contribute to birth-related pulmonary vasodilation in fetal lambs.