Third ventricular pineal gland grafts in the gerbil: an electron microscopical and immunohistochemical investigation.
Third ventricular pineal gland grafts in the gerbil: an electron microscopical and immunohistochemical investigation.
复制标题
沙鼠第三脑室松果体移植物：电子显微镜和免疫组织化学研究。
DOI：
10.1002/jemt.1070210304
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发表时间：
1992
影响因子：
2.5
通讯作者：
Li,K
中科院分区：
文献类型：
--
作者：
Welsh,MG;Li,K
将松果体浅表体移植到成年蒙古沙鼠的第三脑室。新生沙鼠和3-4周龄沙鼠的供体松果体都能存活至少6个月。移植的浅表松果体的松果体细胞保持了原位松果体复合物的形态和S抗原免疫反应性。突触带和球粒存在，但罕见。与原位松果体细胞不同，移植的松果体细胞中普遍存在糖原积累。当移植物的脑室表面被脑脊液（CSF）覆盖时，结构发生了特定部位的调节，脑脊液（CSF）与深松果体中典型的松果体细胞接触。移植物与脑脊液接触的松果体细胞有许多突起沿着移植物的心室表面延伸。移植物的血管具有非开窗内皮和宽的血管周围区域，这是原位松果体复合体的典型特征。移植物组织中存在酪氨酸羟化酶免疫阳性的神经纤维，表明移植物神经再生。纤维的来源尚未确定。神经纤维既存在于血管周围区域，也存在于与松果体细胞相关的实质内。结果表明脑室是浅表松果体移植存活的理想位置。提示松果体移植可能是进一步研究松果体发育和神经支配的一种手段。©1992 Wiley‐Liss, Inc。
The superficial pineal gland was grafted into the third ventricle of adult mongolian gerbils. Donor pineal glands from both neonatal and 3–4 week old gerbils were able to survive for at least 6 months. The pinealocytes of the grafted superficial pineal glands maintained the morphology and the S‐antigen immunoreactivity of the in situ pineal complex. Synaptic ribbons and spherules were present but rare. Unlike the in situ pinealocytes, glycogen accumulations were common in the graft pinealocytes. Site specific modulation of structure was indicated as the ventricular surface of the grafts became covered with cerebrospinal fluid (CSF)‐contacting pinealocytes typical of those seen in the deep pineal. The CSF‐contacting pinealocytes of the graft had numerous processes that extended along the ventricular surface of the graft. The blood vessels of the grafts had non‐fenestrated endothelium and wide perivascular areas typical of those seen in the in situ pineal complex. Tyrosine hydroxylase‐immunopositive nerve fibers were present in the grafted tissue indicating reinnervation of the graft. The source of the fibers was not determined. The nerve fibers were present both within the perivascular area and within the parenchyma where they were associated with pinealocytes. The results demonstrate that the cerebral ventricles are an ideal location for the survival of superficial pineal gland grafts. It is suggested that pineal grafts may be a means to further study pineal development and innervation. © 1992 Wiley‐Liss, Inc.