Quantification of spatial parameters in 3D cellular constructs using graph theory.
Quantification of spatial parameters in 3D cellular constructs using graph theory.
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DOI：
10.1155/2009/928286
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发表时间：
2009
影响因子：
--
通讯作者：
Plopper GE
中科院分区：
文献类型：
--
作者：
Lund AW;Bilgin CC;Hasan MA;McKeen LM;Stegemann JP;Yener B;Zaki MJ;Plopper GE
多光谱三维成像为传统方法无法测量的生物结构提供了空间信息。这项工作提出了一种跟踪三维生物结构的方法，利用图论来量化随时间的变化。细胞图是根据胶原凝胶压实过程中细胞核之间的成对距离在三维欧几里得空间中生成的。从这些图中提取定量特征，测量全球地形和频繁出现的“组织结构”的局部结构。特征趋势可以通过操纵单元密度的压缩来控制，并且与随机图相比是重要的。这项工作提出了一种新的方法来跟踪一个简单的3D生物事件和定量分析潜在的结构变化。该方法的进一步应用将允许研究复杂的生物问题，这些问题需要在3D中量化时空信息，并在理解结构-功能关系方面建立新的范例。
Multispectral three-dimensional (3D) imaging provides spatial information for biological structures that cannot be measured by traditional methods. This work presents a method of tracking 3D biological structures to quantify changes over time using graph theory. Cell-graphs were generated based on the pairwise distances, in 3D-Euclidean space, between nuclei during collagen I gel compaction. From these graphs quantitative features are extracted that measure both the global topography and the frequently occurring local structures of the “tissue constructs.” The feature trends can be controlled by manipulating compaction through cell density and are significant when compared to random graphs. This work presents a novel methodology to track a simple 3D biological event and quantitatively analyze the underlying structural change. Further application of this method will allow for the study of complex biological problems that require the quantification of temporal-spatial information in 3D and establish a new paradigm in understanding structure-function relationships.
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影响因子：
5.8
作者：
Demir, C;Gultekin, SH;Yener, B
通讯作者：
Yener, B
DOI：
10.1073/pnas.0705081104
发表时间：
2007-11-13
影响因子：
11.1
作者：
Park, Juyong;Barabasi, Albert-Laszlo
通讯作者：
Barabasi, Albert-Laszlo
影响因子：
4
作者：
Gulbahce, Natali;Lehmann, Sune
通讯作者：
Lehmann, Sune
影响因子：
2
作者：
Fricker, M. D.;Lee, J. A.;Boddy, L.
通讯作者：
Boddy, L.
影响因子：
5.8
作者：
Gunduz, Cigdem;Yener, Buelent;Gultekin, S. Humayun
通讯作者：
Gultekin, S. Humayun