A PerR-like protein involved in response to oxidative stress in the extreme bacterium Deinococcus radiodurans
A PerR-like protein involved in response to oxidative stress in the extreme bacterium Deinococcus radiodurans
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一种类似 PerR 的蛋白参与极端细菌耐辐射奇球菌的氧化应激反应
DOI：
10.1016/j.bbrc.2014.06.015
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发表时间：
2014
影响因子：
3.1
通讯作者：
Hua Yuejin
中科院分区：
文献类型：
--
作者：
Liu Chengzhi;Wang Liangyan;Li Tao;Lin Lin;Dai Shang;Tian Bing;Hua Yuejin
对活性氧（ROS）的反应和防御系统有助于耐辐射球菌对氧化剂或辐射引起的氧化应激具有显著的抗性。然而，涉及ROS反应和防御系统的机制。放射性细菌还没有被很好地理解。皮毛家族蛋白在ROS反应中起重要作用。目前仅能通过序列相似性预测单一的毛皮同源物。radioduransgenome数据库。我们的生物信息学分析表明，在d的基因组中有一个额外的鸟嘌呤核苷酸。这导致了另一种与Fur同源的DrPerR的发现。基因破坏突变体DrPerR对过氧化氢（H2O2）的抗性增强，细胞提取物中过氧化氢酶活性增加。Real-time PCR结果表明，DrPerR是过氧化氢酶基因kate的抑制因子。同时，DrPerR对h2o2胁迫下饥饿细胞dna结合蛋白（dps）基因的抑制表明其在金属离子止血中的调控作用。因此，DrPerR可能是Fur的同源蛋白，参与了ROS的应答和防御。这些结果有助于阐明对活性氧应激的复杂调控网络。
Response and defense systems against reactive oxygen species (ROS) contribute to the remarkable resistance ofDeinococcus radioduransto oxidative stress induced by oxidants or radiation. However, mechanisms involved in ROS response and defense systems ofD. radioduransare not well understood. Fur family proteins are important in ROS response. Only a single Fur homolog is predicted by sequence similarity in the currentD. radioduransgenome database. Our bioinformatics analysis demonstrated an additional guanine nucleotide in the genome ofD. radioduransthat is not in the database, leading to the discovery of another Fur homolog DrPerR. Gene disruption mutant of DrPerR showed enhanced resistance to hydrogen peroxide (H2O2) and increased catalase activity in cell extracts. Real-time PCR results indicated that DrPerR functions as a repressor of the catalase genekatE.Meanwhile, derepression ofdps(DNA-binding proteins from starved cells) gene under H2O2stress by DrPerR point to its regulatory role in metal ions hemostasis. Thus, DrPerR might function as a Fur homolog protein which is involved in ROS response and defense. These results help clarify the complicated regulatory network that responds to ROS stress inD. radiodurans.