Glucose-6-phosphate dehydrogenase activity in somatic and germinal cells of the mouse testis.
Glucose-6-phosphate dehydrogenase activity in somatic and germinal cells of the mouse testis.
复制标题
小鼠睾丸体细胞和生殖细胞中的葡萄糖-6-磷酸脱氢酶活性。
DOI：
10.1530/jrf.0.0540357
复制
发表时间：
1978
期刊：
影响因子：
--
通讯作者：
S. Andrews
中科院分区：
文献类型：
--
作者：
J. T. Jones;S. Andrews
用分光光度法和电泳法检测了不同日龄（5-62天）小鼠睾丸细胞组分中G6 PD的活性。(1)曲细精管细胞的G6 PD活性与间质细胞的G6 PD活性相当或更高。(2)未成熟小鼠睾丸的高G6 PD活性主要是由于随着年龄的增长而下降的支持细胞的高比活性。(3)在培养过程中，从生殖细胞比从间质细胞或支持细胞更容易失去G6 PD活性。(4)所有的睾丸细胞类型都含有G6 PD和6-磷酸己糖脱氢酶。目前尚不清楚未成熟小鼠支持细胞中高G6 PD活性的功能意义。
The activity of G6PD was examined by spectrophotometric and electrophoretic assays in cell fractions from the testes of mice at different ages (5-62 days). (1) The G6PD activity of the cells of the seminiferous tubule was as high or higher than that of the interstitial tissue. (2) The high G6PD activity of the testis of the immature mouse was mainly due to a high specific activity of the Sertoli cells which declined with age. (3) The G6PD activity was more easily lost during culture from the germinal cells than from the interstitial or Sertoli cells. (4) All the cell types of the testis contained G6PD and hexose-6-phosphate dehydrogenase. The functional significance of the high G6PD activity in the Sertoli cells of immature mice is not at present understood.