RECK inhibits cervical cancer cell migration and invasion by promoting p53 signaling pathway
RECK inhibits cervical cancer cell migration and invasion by promoting p53 signaling pathway
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DOI：
10.1002/jcb.26441
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发表时间：
2018-04-01
影响因子：
4
通讯作者：
Song, Hongjuan
中科院分区：
文献类型：
--
作者：
Liu, Yuan;Li, Lei;Song, Hongjuan
本研究旨在探讨RECK对宫颈癌细胞迁移和侵袭的影响，以帮助理解相关的分子机制。分别采用QRT-PCR和western blot检测RECK在宫颈癌细胞系HELA和C33 A及正常细胞系H8中的转录和翻译水平。转染RECK过表达载体后，分别采用qRT-PCR和western blot检测RECK mRNA、RECK和p53信号通路相关蛋白（p21、p53、bcl-2和Bax）在宫颈癌细胞中的表达。伤口愈合实验和transwell实验检测转染后宫颈癌细胞迁移情况。RECK在宫颈癌细胞系中的表达明显低于正常细胞系。创伤愈合实验结果表明RECK可抑制宫颈癌细胞的迁移，transwell实验结果表明RECK过表达可抑制宫颈癌细胞的侵袭。Western blot结果表明，RECK的过表达可通过影响p53信号通路相关蛋白的表达而促进p53信号通路的激活，而PFT-1抑制RECK的过表达可拮抗RECK对宫颈癌细胞迁移和侵袭能力的影响。RECK可通过激活p53信号通路抑制宫颈癌细胞的迁移和侵袭。
The present study was conducted to investigate the effects of RECK on cervical cancer cell migration and invasion to help understand relevant molecular mechanisms. QRT-PCR and western blot were respectively utilized to examine the transcriptional and translational levels of RECK in cervical cancer cell lines (HELA and C33A) and normal cell line (H8). After transfection with RECK overexpressing vectors, the expression of RECK mRNA, RECK and p53 signaling pathway-related proteins (p21, p53, bcl-2, and Bax) in cervical cancer cells were respectively examined using qRT-PCR and western blot. Cervical cancer cell migration after transfection was detected by wound healing assay and transwell assay. RECK expression was much lower in cervical cancer cell lines compared with normal cell line. Results of wound-healing assay results indicated that RECK could inhibit cervical cancer cell migration, and transwell assay results demonstrated that cell invasion was suppressed by RECK overexpression. Furthermore, western blot indicated that the overexpression of RECK could promote the activation of p53 signaling pathway by influencing related protein expression; whereas its inhibition by PFT- could antagonize the effect of RECK on migrative and invasive abilities of cervical cancer cells. RECK could inhibit the migration and invasion of cervical cancer cells by activating p53 signaling pathway.