Molecular microbial diversity in a nitrifying reactor system without sludge retention
Molecular microbial diversity in a nitrifying reactor system without sludge retention
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DOI：
10.1111/j.1574-6941.1998.tb00540.x
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发表时间：
1998-11-01
影响因子：
4.2
通讯作者：
Jetten, M
中科院分区：
文献类型：
--
作者：
Logemann, S;Schantl, J;Jetten, M
近年来，单反应器高活性脱氮工艺（SHARON）被开发用于去除高浓度氨氮废水。与常规系统相比，该硝化反应器系统在相对高的温度（35摄氏度）下操作而没有污泥滞留。描述反应器中存在的微生物群落的经典方法失败了，因此，使用几种互补的分子生物学技术研究了在该单一反应器系统中负责氨去除的微生物。通过这些分子方法获得的结果是在良好的协议彼此，并证明成功的监测微生物多样性。16S rRNA PCR产物的变性梯度凝胶电泳被证明是一种有效的技术，以快速估计至少四种不同类型的细菌在SHARON反应器中的存在。此外，16S rRNA基因文库分析表明，有一个占优势的克隆（69%），这是高度相似的亚硝化单胞菌真养型（98.8%）。在其他克隆中，14%可以被分配到噬细胞杆菌组的新成员。这些数据通过两种独立的显微镜方法进行了定性和定量证实。使用针对亚硝化单胞菌簇的16S rRNA的荧光寡核苷酸探针（NEU）证明了约70%的氨氧化细菌的存在。电子显微镜照片显示了SHARON反应堆大部分电池中氨氧化剂的典型形态。(C)1998年，欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
Recently, the single reactor system for high activity ammonia removal over nitrite (SHARON) process was developed for the removal of ammonia from wastewater with high ammonia concentrations. In contrast to normal systems, this nitrifying reactor system is operated at relatively high temperatures (35 degrees C) without sludge retention. Classical methods to describe the microbial community present in the reactor failed and, therefore, the microorganisms responsible for ammonia removal in this single reactor system were investigated using several complementary molecular biological techniques. The results obtained via these molecular methods were in good agreement with each other and demonstrated successful monitoring of microbial diversity. Denaturing gradient gel electrophoresis of 16S rRNA PCR products proved to be an effective technique to estimate rapidly the presence of at least four different types of bacteria in the SHARON reactor. In addition, analysis of a 16S rRNA gene library revealed that there was one dominant (69%) clone which was highly similar (98.8%) to Nitrosomonas eutropha. Of the other clones, 14% could be assigned to new members of the CytophagalFlexibacter group. These data were qualitatively and quantitatively confirmed by two independent microscopic methods. The presence of about 70% ammonia oxidizing bacteria was demonstrated using a fluorescent oligonucleotide probe (NEU) targeted against the 16S rRNA of the Nitrosomonas cluster. Electron microscopic pictures showed the typical morphology of ammonia oxidizers in the majority of the cells from the SHARON reactor. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.