Diversity of oral asaccharolytic Eubacterium species in periodontitis--identification of novel phylotypes representing uncultivated taxa.
Diversity of oral asaccharolytic Eubacterium species in periodontitis--identification of novel phylotypes representing uncultivated taxa.
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牙周炎中口腔解糖真杆菌物种的多样性——代表未培养类群的新系统型的鉴定。
DOI：
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发表时间：
1999
影响因子：
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通讯作者：
W. Wade
中科院分区：
文献类型：
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作者：
D. Spratt;A. Weightman;W. Wade
口腔溶砷真杆菌属与牙周病和其他口腔感染有关。本研究的目的是使用一种不依赖于培养的分子技术来确定牙周炎龈下菌斑中溶砷真杆菌的多样性。设计了一对寡核苷酸PCR引物，用于扩增口腔溶砷真杆菌系统发育树分支的16SrRNA基因。该引物与PCR中的通用引物一起用于直接从单个龈下菌斑样品扩增基因序列。通过克隆纯化50个PCR产物，进行全序列测定和分子系统发育分析。这些序列被分配到低G + C革兰氏阳性细菌的单一谱系内的四个组。组I（58%的克隆序列）被分配到包括结节真杆菌的分支，组II（22%）被分配到包括虚弱真杆菌的分支。Ⅲ组（8%）与E.在种的水平上，第IV组（12%）是另一个新的分类群，与E. infirmum和E.节点。
Oral asaccharolytic Eubacterium species are associated with periodontal disease and other oral infections. The aim of this study was to use a culture-independent molecular technique to determine the diversity of asaccharolytic Eubacterium species in subgingival plaque in periodontitis. An oligonucleotide PCR primer designed to amplify 16S rRNA genes of the oral asaccharolytic Eubacterium branch of the phylogenetic tree was constructed. This primer was used together with a universal primer in PCRs to amplify gene sequences directly from a single subgingival plaque sample. Fifty PCR products were purified by cloning, fully sequenced and subjected to molecular phylogenetic analysis. The sequences were assigned to four groups within a single lineage of the low G + C gram-positive bacteria. Group I (58% of the cloned sequences) was assigned to a branch that included Eubacterium nodatum, and Group II (22%) to a branch including Eubacterium infirmum. Group III (8%) was distinct from but related to E. infirmum at the species level, and Group IV (12%) was another novel taxon more distantly related to E. infirmum and E. nodatum.