Analysis of bovine selenoprotein P-like protein gene and availability of metal responsive element (MRE) located in its promoter.-猫眼课题宝

牛硒蛋白 P 样蛋白基因的分析及其启动子中金属响应元件 (MRE) 的可用性。

DOI：

10.1016/s0378-1119(97)00369-7

发表时间：

1997

期刊：

Gene

影响因子：

3.5

通讯作者：

中科院分区：

生物学3区

文献类型：

作者：

M. Fujii;K. Saijoh;T. Kobayashi;S. Fujii;M. J. Lee;K. Sumino

硒蛋白P样蛋白与硒蛋白P类似，使用多个TGAs结合硒半胱氨酸，但不作为终止密码子。它还具有富含his - pro结构域和区域差异表达模式的特征。因此，除了硒代谢外，这种蛋白质被认为具有发育功能。本研究分析了硒蛋白p样蛋白基因的结构。该基因由5个外显子组成，5 '侧区包含TATA box、TCF-1-CS、bHLH-CS、γ-IRE-CS、C - myb - cs、C/EBP-CS、HNF-5-CS、MRE2-CS等。TCF-1-CS、c-Myb-CS等基序的存在支持了该蛋白参与细胞成熟的建议。由于MRE2-CS的存在表明该蛋白与硒对重金属中毒的解毒剂作用有关，因此使用牛肾细胞系CKT-1和MDBK检测了该基元的可用性。10−6M cdcl2和zncl2处理后6h，两种细胞系金属硫蛋白mRNA均显著升高。硒蛋白p样蛋白mRNA未见明显变化，但其基础表达量高，表明该蛋白是组成性表达的。因此，这种蛋白质仍然有可能作为一种解毒剂，即使它不能被重金属诱导。

Selenoprotein P-like protein, similar to selenoprotein P, uses multiple TGAs for incorporation of selenocysteines but not as stop codons. It is also characterized by having a His–Pro-rich domain and a regionally differential expression pattern. Hence, in addition to selenium metabolism, this protein is considered to have a developmental function. In the present study, the structure of the selenoprotein P-like protein gene was analyzed. The gene consisted of five exons, and the 5′-flanking region contained a TATA box, TCF-1-CS, bHLH-CS, γ-IRE-CS, c-Myb-CS, C/EBP-CS, HNF-5-CS, MRE2-CS, etc. The presence of motifs like TCF-1-CS, c-Myb-CS, etc. supports the suggestion that this protein is involved in cellular maturation. Since the presence of MRE2-CS suggests that this protein is related to the antidote effect of selenium against heavy metal intoxication, the availability of this motif was examined using bovine kidney cell lines, CKT-1 and MDBK. Metallothionein mRNA markedly increased 6h after administration of 10−6M CdCl2and ZnCl2in both cell lines. No significant alteration was observed in selenoprotein P-like protein mRNA, whereas its basal expression was high, indicating that this protein is constitutively expressed. Thus, it is still possible that this protein acts as an antidote, even though it is not inducible by heavy metals.