Association of IL1B polymorphisms with gastric cancer in a Chinese population.
Association of IL1B polymorphisms with gastric cancer in a Chinese population.
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DOI：
10.1016/j.clinbiochem.2006.10.018
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发表时间：
2007-02
影响因子：
2.8
通讯作者：
Wei-peng Wang;Kunyi Ni;Guohua Zhou
中科院分区：
文献类型：
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作者：
Wei-peng Wang;Kunyi Ni;Guohua Zhou
目的探讨IL 1B基因多态性与胃癌发病的关系，选择IL 1B基因上的7个SNPs，建立基于接头连接和等位基因特异性扩增的多重基因分型方法，同时对IL 1B基因上的7个SNPs进行分型。对141例非肿瘤门诊患者和97例胃癌患者进行基因分型，分析IL 1B基因多态性与胃癌的关系。结果成功分型出7个SNPs，与桑格测序法和PCR-RFLP法结果一致。经统计学处理，发现0794 C>T（χ2=6.11，P=0.05）、1274 C>T（χ2=6.86，P=0.03）和2143 T>C（χ2=6.86，P=0.03）基因型频率在患者组和对照组间差异有统计学意义。SNPs 0794 C>T、1274 C>T和2143 T>C与胃癌相关。
OBJECTIVESTo investigate the association between IL1B polymorphisms and risk of gastric cancer in a Chinese population, seven SNPs in the IL1B gene were selected for this study.METHODSA multiplex genotyping method, which is based on adapter ligation and allele-specific amplification, was established to type seven SNPs on the IL1B gene simultaneously. One hundred and forty-one non-cancer outpatients and 97 patients with gastric cancer were genotyped, and the relation between IL1B polymorphisms and gastric cancer was statistically analyzed.RESULTSThe seven SNPs were successfully typed and the results were consistent with those obtained by both Sanger's sequencing and PCR-RFLP. Handling with statistical analysis, we observed significantly different genotype frequencies of 0794C>T (χ2=6.11, P=0.05), 1274C>T (χ2=6.86, P=0.03) and 2143T>C (χ2=6.86, P=0.03) between patients and controls.CONCLUSIONALM-ASA is a potential method for multiplex SNP typing with a low consumption of genomic DNA. SNPs 0794C>T, 1274C>T, and 2143T>C are associated with gastric cancer.