VALIDATION OF 2 INVITRO TEST SYSTEMS FOR ESTROGENIC ACTIVITIES WITH ZEARALENONE, PHYTOESTROGENS AND CEREAL EXTRACTS
VALIDATION OF 2 INVITRO TEST SYSTEMS FOR ESTROGENIC ACTIVITIES WITH ZEARALENONE, PHYTOESTROGENS AND CEREAL EXTRACTS
复制标题
DOI：
10.1016/0300-483x(92)90134-z
复制
发表时间：
1992-09-01
期刊：
影响因子：
4.5
通讯作者：
SCHNEIDER, S
中科院分区：
文献类型：
--
作者：
MAYR, U;BUTSCH, A;SCHNEIDER, S
为了建立替代常用的子宫试验与小鼠，确定雌激素敏感的细胞系（MCF-7细胞和LEC-9细胞）被用来确定植物来源（真菌和植物雌激素）和玉米赤霉烯酮污染的饲料谷物（小麦，大麦和燕麦）的纯化化合物的雌激素活性。在人乳腺癌细胞系MCF-7细胞中，雌激素特异性外蛋白的诱导作为雌激素活性的参数。LeC-9细胞代表源自小鼠L细胞的遗传转化细胞克隆。在这里，通过细菌氯霉素乙酰转移酶（CAT）基因在雌激素响应元件的控制下的表达来测量细菌氯霉素样活性。在该系统中，通过第二个报告基因（由组成型人β-肌动蛋白启动子控制的细菌β-半乳糖苷酶基因）的表达监测影响细胞活力的毒性作用。真菌和植物雌激素的相对雌激素活性测定与这两个系统是伴随的，但更高的子宫试验与小鼠相比。
In order to establish alternatives to the frequently used uterotropic assay with mice, defined estrogen-sensitive cell lines (MCF-7 cells and LeC-9 cells) were used to determine the estrogenic activities of purified compounds of vegetable origin (myco- and phytoestrogens) and zearalenone-contaminated forage cereals (wheat, barley and oats). In MCF-7 cells, a human breast cancer cell line, the induction of an estrogen-specific exoprotein served as a parameter of estrogenic activities. LeC-9 cells represent a genetically transformed cell clone derived from mouse L-cells. Here, hormone-like activities were measured by the expression of the bacterial chloramphenicol acetyltransferase (CAT) gene under the control of an estrogen-responsive element. Toxic effects affecting cell viability were monitored in this system by the expression of a second reporter gene (the bacterial beta-galactosidase gene controlled by the constitutive human beta-actin promoter). Relative estrogenic activities of myco- and phytoestrogens determined with both systems are concomitant, but higher as compared to the uterotropic assay with mice.