Acridine sensitivity of bacteriophage T2: a virus gene affecting cell permeability.
Acridine sensitivity of bacteriophage T2: a virus gene affecting cell permeability.
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噬菌体 T2 的吖啶敏感性：影响细胞通透性的病毒基因。
DOI：
10.1016/0022-2836(67)90190-8
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发表时间：
1967
影响因子：
5.6
通讯作者：
S. Silver
中科院分区：
文献类型：
--
作者：
S. Silver
对感染噬菌体 T2 的大肠杆菌细胞摄取吖啶染料的研究表明，染料作为噬菌体增殖抑制剂的效力与噬菌体感染细胞吸收的染料量之间存在相关性。被噬菌体感染的细胞吸收的原黄素大约是纯化吖啶黄素的三分之一。还有少量其他吖啶染料如9-氨基吖啶和奎纳克林被吸收。染料摄取的特异性不是由于选择性渗透性（如热或甲苯破坏细胞的渗透性屏障的实验所示），而是由于细胞结合底物对染料的选择性吸收。Hessler (1963) 研究发现，吖啶黄素的摄取受病毒基因控制。在噬菌体感染后的最初几分钟内，变得可渗透的过程需要能量和蛋白质合成，但在此期间不需要吖啶黄。早期蛋白质合成后，染料吸收过程可以不受氰化物和碘乙酸盐等能量毒物的影响进行。这表明染料的摄取不是通过噬菌体诱导的吖啶泵而是被动的。甲苯实验反驳了噬菌体基因控制的吖啶黄结合底物是由早期蛋白质合成产生的可能性，甲苯实验表明细胞的染料结合能力并未受到噬菌体感染的很大影响。 Prgene 似乎可以改变细胞膜对染料的渗透性。
Studies on the uptake of acridine dyes byEscherichia colicells infected with bacteriophage T2 show a correlation between the potency of the dye as an inhibitor of bacteriophage multiplication and the amount of dye absorbed by the phage-infected cells. About one-third as much proflavine as purified acriflavine is taken up by the phage-infected cells. Still smaller amounts of other acridine dyes such as 9-aminoacridine and quinacrine are absorbed. The specificity of dye uptake is not due to selective permeability (as shown by experiments in which the permeability barriers of the cells are destroyed by heat or toluene) but rather to the selective absorption of the dyes by cellular binding substrates.The uptake of acriflavine is controlled by the viral genepr, studied by Hessler (1963). The process ofbecoming permeablerequires energy and protein synthesis during the first few minutes following phage infection, but the acriflavine need not be present during this period. After early protein synthesis, the process ofdye uptakecan proceed unaffected by energy poisons such as cyanide and iodoacetate. This suggests that the uptake of dye is notviaa phage-induced acridine pump but is passive. The possibility that an acriflavine-binding substrate controlled by a phage gene is produced by the early protein synthesis is argued against by experiments with toluene which show that the dye-binding capacity of the cell is not greatly affected by phage infection. Theprgene appears to alter the permeability of the cell membrane to the dyes.