Association of actin with chromaffin granule membranes and the effect of cytochalasin B on the polarity of actin filament elongation.
Association of actin with chromaffin granule membranes and the effect of cytochalasin B on the polarity of actin filament elongation.
复制标题
肌动蛋白与嗜铬颗粒膜的结合以及细胞松弛素 B 对肌动蛋白丝伸长极性的影响。
DOI：
10.1016/0005-2736(81)90137-1
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Lin,S
中科院分区：
文献类型：
--
作者：
Wilkins,JA;Lin,S
从牛肾上腺髓质分离的嗜铬颗粒的膜显示以高亲和力结合二氢细胞松弛素B。这些膜也结合[3 H]肌动蛋白在时间和Mg 2+依赖的方式和电子显微镜显示存在膜附着的肌动蛋白丝后，加入外源actin.[3 H]肌动蛋白的结合被细胞松弛素B部分抑制。对重肌球蛋白修饰的膜附着细丝的电子显微镜分析显示，末端（末端上）附着的细丝具有两种可能的极性（即，具有指向和远离膜的箭头的细丝）。用细胞松弛素B处理样品优先抑制细丝的生长，其“倒刺”末端指向远离膜。这些结果进行了讨论相对于肌动蛋白的作用，分泌颗粒的功能和细胞松弛素的行动机制。
Membranes of chromaffin granules isolated from bovine adrenal medulla are shown to bind dihydrocytochalasin B with high affinity. These membranes also bound [3H]actin in a time- and Mg2+-dependent manner and electron microscopy showed the presence of membrane-attached actin filaments following addition of exogenous actin.Binding of [3H]actin was partially inhibited by cytochalasin B. Electron microscopic analysis of heavy meromyosin-decorated, membrane-attached filaments showed terminally (end-on) attached filaments with both possible polarities (i.e., filaments with arrowheads pointing both towards and away from the membranes). Treatment of samples with cytochalasin B preferentially inhibited growth of filaments with their ‘barbed’ ends pointing away from membranes. These results are discussed with respect to the role of actin in secretory granule function and the mechanism of cytochalasin action.