Beta-catenin localization during Xenopus embryogenesis: accumulation at tissue and somite boundaries.
Beta-catenin localization during Xenopus embryogenesis: accumulation at tissue and somite boundaries.
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发表时间：
1994-12
期刊：
影响因子：
4.6
通讯作者：
François Fagotto;B. Gumbiner
中科院分区：
文献类型：
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作者：
François Fagotto;B. Gumbiner
-连环蛋白是一种细胞质蛋白，与钙粘蛋白粘附分子相关，并与爪蟾轴的形成有关（McCrea， P. D., Brieher， W. M. and Gumbiner， B. M. (1993) J.细胞生物学，127,477-484）。利用免疫荧光技术对其在爪蟾胚胎中的分布进行了研究。与其在细胞-细胞粘附中的功能一致，β -连环蛋白存在于每个细胞中。然而，高水平表达在胚胎的某些区域和不同组织中。β -连环蛋白的水平与预期的粘附强度之间似乎不存在简单的相关性。高水平的β -连环蛋白存在于形态发生活跃的区域，如囊胚和原胚的边缘区。这表明β -连环蛋白的高表达可能参与了动态粘附事件。令人惊讶的是，β -连环蛋白也会积聚在质膜上，而质膜可能不会与其他细胞建立直接或强烈的接触。特别是，大量的β -连环蛋白在组织胶原之间的边界和体间边界被短暂地发现。这种意想不到的β -连环蛋白表达模式提出了这种分子参与发育过程的可能性，也许独立于其在细胞-细胞粘附中的经典作用。
beta-catenin is a cytoplasmic protein associated with cadherin adhesion molecules and has been implicated in axis formation in Xenopus (McCrea, P. D., Brieher, W. M. and Gumbiner, B. M. (1993) J. Cell Biol. 127, 477-484). We have studied its distribution in Xenopus embryos by immunofluorescence on frozen sections. Consistent with its function in cell-cell adhesion, beta-catenin is present in every cell. However, high levels are expressed in certain regions and different tissues of the embryo. No simple correlation appears to exist between the levels of beta-catenin with the expected strength of adhesion. High levels of beta-catenin were found in regions undergoing active morphogenetic movements, such as the marginal zone of blastulae and gastrulae. This suggests that high expression of beta-catenin could be involved in dynamic adhesion events. Surprisingly, beta-catenin also accumulates on plasma membranes that probably do not establish direct or strong contacts with other cells. In particular, high amounts of beta-catenin are found transiently at boundaries between tissue anlagen and at the intersomitic boundaries. This unexpected pattern of beta-catenin expression raises the possibility that this molecule participates in developmental processes, perhaps independently of its classical role in cell-cell adhesion.