CONTINUOUS GROWTH OF PROXIMAL TUBULAR KIDNEY EPITHELIAL-CELLS IN HORMONE-SUPPLEMENTED SERUM-FREE MEDIUM
CONTINUOUS GROWTH OF PROXIMAL TUBULAR KIDNEY EPITHELIAL-CELLS IN HORMONE-SUPPLEMENTED SERUM-FREE MEDIUM
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DOI：
10.1083/jcb.94.3.506
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发表时间：
1982-01-01
影响因子：
7.8
通讯作者：
SAIER, MH
中科院分区：
文献类型：
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作者：
CHUMAN, L;FINE, LG;SAIER, MH
猪肾上皮细胞系（LLC-PK 1）具有近端肾小管细胞的特性，可以在无血清培养基中无限期维持。持续生长需要7个因子的存在：转铁蛋白、胰岛素、硒、氢化可的松、三碘甲状腺原氨酸、加压素和胆固醇。该培养基支持LLC-PK 1细胞以接近在补充血清的培养基中观察到的速率生长;允许通过半囊形成测量的载体跨上皮盐和流体运输;并影响细胞形态。生长和形态的加压素依赖性可以部分被异丁基甲基黄嘌呤或二丁酰cAMP取代。使用该培养基分离不含成纤维细胞的兔近端肾小管肾上皮细胞。
An epithelial cell line from pig kidney (LLC-PK1) with properties of proximal tubular cells can be maintained indefinitely in hormone-supplemented serum-free medium. Continuous growth requires the presence of 7 factors: transferrin, insulin, Se, hydrocortisone, triiodothyronine, vasopressin and cholesterol. The hormone-defined medium supports growth of LLC-PK1 cells at a rate approaching that observed in serum-supplemented medium; allows vectorial transepithelial salt and fluid transport as measured by hemicyst formation; and influences cell morphology. The vasopressin dependency for growth and morphology can be partially replaced by isobutylmethylxanthine or dibutyryl cAMP. The medium was used to isolate rabit proximal tubular kidney epithelial cells free of fibroblasts.