Growth Inhibition and Cell Cycle Arrest in the G0/G1 by Schizandrin, a Dibenzocyclooctadiene Lignan Isolated from Schisandra chinensis, on T47D Human Breast Cancer Cells
Growth Inhibition and Cell Cycle Arrest in the G0/G1 by Schizandrin, a Dibenzocyclooctadiene Lignan Isolated from Schisandra chinensis, on T47D Human Breast Cancer Cells
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DOI：
10.1002/ptr.2907
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发表时间：
2010-02-01
影响因子：
7.2
通讯作者：
Lee, Sang Kook
中科院分区：
文献类型：
--
作者：
Kim, Sun-Jack;Min, Hye-Young;Lee, Sang Kook
五味子素是五味子果实中主要的二苯并环七烯木脂素之一。五味子甲素和其他二苯并环二烯木脂素具有保肝、抗病毒和神经保护作用。然而，五味子甲素对人类癌细胞的抗增殖作用至今尚未确定。本研究考察了五味子甲素对人乳腺癌细胞的抗增殖作用。五味子甲素在培养的人乳腺癌细胞中表现出抑制生长的活性，并且在雌激素受体（ER）阳性的T47D细胞中的作用比在雌激素受体（ER）阴性的MDA-MB-231细胞中的作用更明显。当在T47D细胞中使用该化合物时，五味子甲素诱导G0/G1期细胞群的积累，这进一步通过诱导CDK抑制剂p21和p27以及抑制细胞周期检查点蛋白cyclin D1、cyclin a、CDK2和CDK4的表达得到证实。这些结果表明，五味子甲素通过调节人类乳腺癌细胞周期相关蛋白诱导细胞周期阻滞来抑制细胞增殖。版权所有John Wiley & Sons， Ltd. 2009
Schizandrin is one of the main dibenzocyclooctadiene lignans present in the fruit of Schisandra chinensis (Schisandraceae). Biological activities including hepatoprotective, antiviral and neuroprotective effects of schizandrin and other dibenzocyclooctadiene lignans have been reported previously. However, the antiproliferative effect of schizandrin against human cancer cells has been poorly determined to date. This study examined the antiproliferative effect of schizandrin in human breast cancer cells. Schizandrin exhibited growth inhibitory activities in cultured human breast cancer cells, and the effect was the more profound in estrogen receptor (ER)-positive T47D cells than in ER-negative MDA-MB-231 cells. When treated with the compound in T47D cells, schizandrin induced the accumulation of a cell population in the G0/G1 phase, which was further demonstrated by, the induction of CDK inhibitors p21 and p27 and the inhibition of the expression of cell cycle checkpoint proteins including cyclin D1, cyclin A, CDK2 and CDK4. These results suggest that schizandrin inhibits cell proliferation through the induction of cell cycle arrest with modulating cell cycle-related proteins in human breast cancer cells. Copyright (C) 2009 John Wiley & Sons, Ltd.